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Nat Methods DOI:

Cell type–specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function.

Publication TypeJournal Article
Year of Publication2011
AuthorsZhao, S, Ting, JT, Atallah, HE, Qiu, L, Tan, J, Gloss, B, Augustine, GJ, Deisseroth, K, Luo, M, Graybiel, AM, Feng, G
JournalNat Methods
Date Published2011 Sep
KeywordsAction Potentials, Animals, Choline O-Acetyltransferase, Chromosomes, Artificial, Bacterial, Hippocampus, Mice, Mice, Transgenic, Nerve Tissue, Neurons, Rhodopsin, Tryptophan Hydroxylase, Vesicular Inhibitory Amino Acid Transport Proteins

Optogenetic methods have emerged as powerful tools for dissecting neural circuit connectivity, function and dysfunction. We used a bacterial artificial chromosome (BAC) transgenic strategy to express the H134R variant of channelrhodopsin-2, ChR2(H134R), under the control of cell type–specific promoter elements. We performed an extensive functional characterization of the newly established VGAT-ChR2(H134R)-EYFP, ChAT-ChR2(H134R)-EYFP, Tph2-ChR2(H134R)-EYFP and Pvalb(H134R)-ChR2-EYFP BAC transgenic mouse lines and demonstrate the utility of these lines for precisely controlling action-potential firing of GABAergic, cholinergic, serotonergic and parvalbumin-expressing neuron subsets using blue light. This resource of cell type–specific ChR2(H134R) mouse lines will facilitate the precise mapping of neuronal connectivity and the dissection of the neural basis of behavior.


Alternate JournalNat. Methods
PubMed ID21985008
PubMed Central IDPMC3191888
Grant ListRC1 MH088434 / MH / NIMH NIH HHS / United States
R01 MH060379 / MH / NIMH NIH HHS / United States
RC1 MH088434-01 / MH / NIMH NIH HHS / United States
F32MH084460 / MH / NIMH NIH HHS / United States
RC1-MH088434 / MH / NIMH NIH HHS / United States
F32 MH084460 / MH / NIMH NIH HHS / United States