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Neuron DOI:10.1016/j.neuron.2012.07.011

Imaging neural activity using Thy1-GCaMP transgenic mice.

Publication TypeJournal Article
Year of Publication2012
AuthorsChen, Q, Cichon, J, Wang, W, Qiu, L, Lee, S-JR, Campbell, NR, Destefino, N, Goard, MJ, Fu, Z, Yasuda, R, Looger, LL, Arenkiel, BR, Gan, W-B, Feng, G
Date Published2012 Oct 18
KeywordsAge Factors, Animals, Antigens, Thy-1, Biophysics, Brain, Calcium, Calmodulin, Cell Count, Cell Line, Transformed, Dendrites, Dose-Response Relationship, Drug, Electric Stimulation, Gene Expression Regulation, Green Fluorescent Proteins, Humans, In Vitro Techniques, Membrane Potentials, Mice, Mice, Inbred C57BL, Mice, Transgenic, Microscopy, Confocal, Mutation, Myosin-Light-Chain Kinase, Neurons, Odorants, Patch-Clamp Techniques, Peptide Fragments, Potassium Chloride, Retina, Transfection

The ability to chronically monitor neuronal activity in the living brain is essential for understanding the organization and function of the nervous system. The genetically encoded green fluorescent protein-based calcium sensor GCaMP provides a powerful tool for detecting calcium transients in neuronal somata, processes, and synapses that are triggered by neuronal activities. Here we report the generation and characterization of transgenic mice that express improved GCaMPs in various neuronal subpopulations under the control of the Thy1 promoter. In vitro and in vivo studies show that calcium transients induced by spontaneous and stimulus-evoked neuronal activities can be readily detected at the level of individual cells and synapses in acute brain slices, as well as chronically in awake, behaving animals. These GCaMP transgenic mice allow investigation of activity patterns in defined neuronal populations in the living brain and will greatly facilitate dissecting complex structural and functional relationships of neural networks.


Alternate JournalNeuron
PubMed ID23083733
PubMed Central IDPMC4059513
Grant ListNS047325 / NS / NINDS NIH HHS / United States
R01 NS078294 / NS / NINDS NIH HHS / United States
R00 NS064171 / NS / NINDS NIH HHS / United States
R01 NS047325 / NS / NINDS NIH HHS / United States
T32 GM007753 / GM / NIGMS NIH HHS / United States
R00NS64171 / NS / NINDS NIH HHS / United States
R01NS078294 / NS / NINDS NIH HHS / United States