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Curr Biol DOI:10.1016/j.cub.2014.02.011

Selective activation of cholinergic basal forebrain neurons induces immediate sleep-wake transitions.

Publication TypeJournal Article
Year of Publication2014
AuthorsHan, Y, Shi, Y-feng, Xi, W, Zhou, R, Tan, Z-bing, Wang, H, Li, X-ming, Chen, Z, Feng, G, Luo, M, Huang, Z-li, Duan, S, Yu, Y-qin
JournalCurr Biol
Volume24
Issue6
Pages693-8
Date Published2014 Mar 17
ISSN1879-0445
KeywordsAction Potentials, Animals, Cholinergic Neurons, Electroencephalography, Electromyography, Mice, Transgenic, Photic Stimulation, Prosencephalon, Rhodopsin, Sleep, Sleep, REM, Wakefulness
Abstract

The basal forebrain (BF) plays a crucial role in cortical activation [1, 2]. However, the exact role of cholinergic BF (ch-BF) neurons in the sleep-wake cycle remains unclear [3, 4]. We demonstrated that photostimulation of ch-BF neurons genetically targeted with channelrhodopsin 2 (ChR2) was sufficient to induce an immediate transition to waking or rapid eye movement (REM) sleep from slow-wave sleep (SWS). Light stimulation was most likely to induce behavioral arousal during SWS, but not during REM sleep, a result in contrast to the previously reported photostimulation of noradrenergic or hypocretin neurons that induces wake transitions from both SWS and REM sleep. Furthermore, the ratio of light-induced transitions from SWS to wakefulness or to REM sleep did not significantly differ from that of natural transitions, suggesting that activation of ch-BF neurons facilitates the transition from SWS but does not change the direction of the transition. Excitation of ch-BF neurons during wakefulness or REM sleep sustained the cortical activation. Stimulation of these neurons for 1 hr induced a delayed increase in the duration of wakefulness in the subsequent inactive period. Our results suggest that activation of ch-BF neurons alone is sufficient to suppress SWS and promote wakefulness and REM sleep.

DOI10.1016/j.cub.2014.02.011
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/24613308?dopt=Abstract

Alternate JournalCurr. Biol.
PubMed ID24613308