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J Neurosci DOI:10.1523/JNEUROSCI.4366-15.2016

Optogenetic Visualization of Presynaptic Tonic Inhibition of Cerebellar Parallel Fibers.

Publication TypeJournal Article
Year of Publication2016
AuthorsBerglund, K, Wen, L, Dunbar, RL, Feng, G, Augustine, GJ
JournalJ Neurosci
Volume36
Issue21
Pages5709-23
Date Published2016 May 25
ISSN1529-2401
KeywordsAction Potentials, Animals, Axons, Brain Mapping, Cells, Cultured, Cerebellum, Female, gamma-Aminobutyric Acid, Male, Mice, Mice, Transgenic, Neurotransmitter Agents, Optogenetics, Presynaptic Terminals, Synaptic Transmission, Voltage-Sensitive Dye Imaging
Abstract

UNLABELLED: Tonic inhibition was imaged in cerebellar granule cells of transgenic mice expressing the optogenetic chloride indicator, Clomeleon. Blockade of GABAA receptors substantially reduced chloride concentration in granule cells due to block of tonic inhibition. This indicates that tonic inhibition is a significant contributor to the resting chloride concentration of these cells. Tonic inhibition was observed not only in granule cell bodies, but also in their axons, the parallel fibers (PFs). This presynaptic tonic inhibition could be observed in slices both at room and physiological temperatures, as well as in vivo, and has many of the same properties as tonic inhibition measured in granule cell bodies. GABA application revealed that PFs possess at least two types of GABAA receptor: one high-affinity receptor that is activated by ambient GABA and causes a chloride influx that mediates tonic inhibition, and a second with a low affinity for GABA that causes a chloride efflux that excites PFs. Presynaptic tonic inhibition regulates glutamate release from PFs because GABAA receptor blockade enhanced both the frequency of spontaneous EPSCs and the amplitude of evoked EPSCs at the PF-Purkinje cell synapse. We conclude that tonic inhibition of PFs could play an important role in regulating information flow though cerebellar synaptic circuits. Such cross talk between phasic and tonic signaling could be a general mechanism for fine tuning of synaptic circuits.

SIGNIFICANCE STATEMENT: This paper demonstrates that an unconventional form of signaling, known as tonic inhibition, is found in presynaptic terminals and affects conventional synaptic communication. Our results establish the basic characteristics and mechanisms of presynaptic tonic inhibition and show that it occurs in vivo as well as in isolated brain tissue.

DOI10.1523/JNEUROSCI.4366-15.2016
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/27225762?dopt=Abstract

Alternate JournalJ. Neurosci.
PubMed ID27225762
PubMed Central IDPMC4879193
Grant ListU01 MH106013 / MH / NIMH NIH HHS / United States