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Nat Neurosci DOI:10.1038/nn.4273

Modeling ALS with motor neurons derived from human induced pluripotent stem cells.

Publication TypeJournal Article
Year of Publication2016
AuthorsSances, S, Bruijn, LI, Chandran, S, Eggan, K, Ho, R, Klim, JR, Livesey, MR, Lowry, E, Macklis, JD, Rushton, D, Sadegh, C, Sareen, D, Wichterle, H, Zhang, S-C, Svendsen, CN
JournalNat Neurosci
Volume19
Issue4
Pages542-53
Date Published2016 Apr
ISSN1546-1726
KeywordsAmyotrophic Lateral Sclerosis, Animals, Cell Differentiation, Cells, Cultured, Coculture Techniques, Humans, Induced Pluripotent Stem Cells, Motor Neurons
Abstract

Directing the differentiation of induced pluripotent stem cells into motor neurons has allowed investigators to develop new models of amyotrophic lateral sclerosis (ALS). However, techniques vary between laboratories and the cells do not appear to mature into fully functional adult motor neurons. Here we discuss common developmental principles of both lower and upper motor neuron development that have led to specific derivation techniques. We then suggest how these motor neurons may be matured further either through direct expression or administration of specific factors or coculture approaches with other tissues. Ultimately, through a greater understanding of motor neuron biology, it will be possible to establish more reliable models of ALS. These in turn will have a greater chance of validating new drugs that may be effective for the disease.

DOI10.1038/nn.4273
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/27021939?dopt=Abstract

Alternate JournalNat. Neurosci.
PubMed ID27021939
PubMed Central IDPMC5015775
Grant ListR01 NS049553 / NS / NINDS NIH HHS / United States
R37NS041590 / NS / NINDS NIH HHS / United States
R37 NS041590 / NS / NINDS NIH HHS / United States
R01 NS041590 / NS / NINDS NIH HHS / United States
R01NS045523 / NS / NINDS NIH HHS / United States
R01NS075672 / NS / NINDS NIH HHS / United States
P30 DK063491 / DK / NIDDK NIH HHS / United States
R01 NS045523 / NS / NINDS NIH HHS / United States
R01NS049553 / NS / NINDS NIH HHS / United States
R01 NS075672 / NS / NINDS NIH HHS / United States