Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
Nat Biotechnol
| Authors | |
| Abstract | Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously. |
| Year of Publication | 2017
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| Journal | Nat Biotechnol
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| Volume | 35
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| Issue | 1
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| Pages | 31-34
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| Date Published | 2017 Jan
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| ISSN | 1546-1696
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| DOI | 10.1038/nbt.3737
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| PubMed ID | 27918548
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| PubMed Central ID | PMC5225075
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| Links | |
| Grant list | DP1 MH100706 / MH / NIMH NIH HHS / United States
R01 MH110049 / MH / NIMH NIH HHS / United States
T32 EB001680 / EB / NIBIB NIH HHS / United States
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