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Nat Neurosci DOI:10.1038/nn.4430

A viral strategy for targeting and manipulating interneurons across vertebrate species.

Publication TypeJournal Article
Year of Publication2016
AuthorsDimidschstein, J, Chen, Q, Tremblay, R, Rogers, SL, Saldi, G-A, Guo, L, Xu, Q, Liu, R, Lu, C, Chu, J, Grimley, JS, Krostag, A-R, Kaykas, A, Avery, MC, Rashid, MS, Baek, M, Jacob, AL, Smith, GB, Wilson, DE, Kosche, G, Kruglikov, I, Rusielewicz, T, Kotak, VC, Mowery, TM, Anderson, SA, Callaway, EM, Dasen, JS, Fitzpatrick, D, Fossati, V, Long, MA, Noggle, S, Reynolds, JH, Sanes, DH, Rudy, B, Feng, G, Fishell, G
JournalNat Neurosci
Volume19
Issue12
Pages1743-1749
Date Published2016 Dec
ISSN1546-1726
KeywordsAnimals, Behavior, Animal, Brain, Cells, Cultured, Dependovirus, Female, GABAergic Neurons, Genetic Vectors, Interneurons, Mice, Inbred C57BL, Vertebrates
Abstract

A fundamental impediment to understanding the brain is the availability of inexpensive and robust methods for targeting and manipulating specific neuronal populations. The need to overcome this barrier is pressing because there are considerable anatomical, physiological, cognitive and behavioral differences between mice and higher mammalian species in which it is difficult to specifically target and manipulate genetically defined functional cell types. In particular, it is unclear the degree to which insights from mouse models can shed light on the neural mechanisms that mediate cognitive functions in higher species, including humans. Here we describe a novel recombinant adeno-associated virus that restricts gene expression to GABAergic interneurons within the telencephalon. We demonstrate that the viral expression is specific and robust, allowing for morphological visualization, activity monitoring and functional manipulation of interneurons in both mice and non-genetically tractable species, thus opening the possibility to study GABAergic function in virtually any vertebrate species.

DOI10.1038/nn.4430
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/27798629?dopt=Abstract

Alternate JournalNat. Neurosci.
PubMed ID27798629
PubMed Central IDPMC5348112
Grant ListP01 NS074972 / NS / NINDS NIH HHS / United States
R01 EY022577 / EY / NEI NIH HHS / United States
R01 MH071679 / MH / NIMH NIH HHS / United States
R01 EY011488 / EY / NEI NIH HHS / United States
R01 MH063912 / MH / NIMH NIH HHS / United States
R01 MH095147 / MH / NIMH NIH HHS / United States
R01 MH066912 / MH / NIMH NIH HHS / United States