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Stem Cell Res DOI:10.1016/j.scr.2016.09.009

Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9.

Publication TypeJournal Article
Year of Publication2016
AuthorsHerring, A, Messana, A, Bara, AM, Hazelbaker, DZ, Eggan, K, Barrett, LE
JournalStem Cell Res
Volume17
Issue2
Pages430-432
Date Published2016 Sep
ISSN1876-7753
Abstract

Here, we generated a biallelic mutation in the TLE1 (Transducin Like Enhancer of Split 1) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The homozygous knockout cell line, TLE1-464-G04, displays loss of TLE1 protein expression while maintaining pluripotency, differentiation potential and genomic integrity.

DOI10.1016/j.scr.2016.09.009
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/27879218?dopt=Abstract

Alternate JournalStem Cell Res
PubMed ID27879218