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Stem Cell Res DOI:10.1016/j.scr.2016.09.008

Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9.

Publication TypeJournal Article
Year of Publication2016
AuthorsBara, AM, Messana, A, Herring, A, Hazelbaker, DZ, Eggan, K, Barrett, LE
JournalStem Cell Res
Volume17
Issue2
Pages441-443
Date Published2016 Sep
ISSN1876-7753
Abstract

Here, we generated a monoallelic mutation in the TLE3 (Transducin Like Enhancer of Split 3) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The heterozygous knockout cell line, TLE3-447-D08-A01, displays partial loss of TLE3 protein expression while maintaining pluripotency, differentiation potential and genomic integrity.

DOI10.1016/j.scr.2016.09.008
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/27879221?dopt=Abstract

Alternate JournalStem Cell Res
PubMed ID27879221