Model-directed generation of artificial CRISPR-Cas13a guide RNA sequences improves nucleic acid detection.

Nature biotechnology
Authors
Abstract

CRISPR guide RNA sequences deriving exactly from natural sequences may not perform optimally in every application. Here we implement and evaluate algorithms for designing maximally fit, artificial CRISPR-Cas13a guides with multiple mismatches to natural sequences that are tailored for diagnostic applications. These guides offer more sensitive detection of diverse pathogens and discrimination of pathogen variants compared with guides derived directly from natural sequences and illuminate design principles that broaden Cas13a targeting.

Year of Publication
2024
Journal
Nature biotechnology
Date Published
10/2024
ISSN
1546-1696
DOI
10.1038/s41587-024-02422-w
PubMed ID
39394482
Links