Publication Type | Journal Article |
Year of Publication | 2022 |
Authors | Weingarten-Gabbay, S, Pearlman, LR, Chen, D-Y, Klaeger, S, Taylor, HB, Welch, NL, Keskin, DB, Carr, SA, Abelin, JG, Saeed, M, Sabeti, PC |
Journal | STAR Protoc |
Volume | 3 |
Issue | 4 |
Pages | 101910 |
Date Published | 2022 Dec 16 |
ISSN | 2666-1667 |
Keywords | Histocompatibility Antigens Class I, Humans, Mass Spectrometry, Peptides, Viruses |
Abstract | Immunopeptidome profiling of infected cells is a powerful technique for detecting viral peptides that are naturally processed and loaded onto class I human leukocyte antigens (HLAs-I). Here, we provide a protocol for preparing samples for immunopeptidome profiling that can inactivate enveloped viruses while still preserving the integrity of the HLA-I complex. We detail steps for lysate preparation of infected cells followed by HLA-I immunoprecipitation and virus inactivation. We further describe peptide purification for mass spectrometry outside a high-containment facility. For complete details on the use and execution of this protocol, please refer to Weingarten-Gabbay et al. (2021).. |
DOI | 10.1016/j.xpro.2022.101910 |
Pubmed | |
Alternate Journal | STAR Protoc |
PubMed ID | 36595954 |
PubMed Central ID | PMC9731565 |
Grant List | U19 AI110818 / AI / NIAID NIH HHS / United States |
STAR Protoc DOI:10.1016/j.xpro.2022.101910
HLA-I immunopeptidome profiling of human cells infected with high-containment enveloped viruses.
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