Development of potent in vivo mutagenesis plasmids with broad mutational spectra.

Nat Commun
Authors
Keywords
Abstract

Methods to enhance random mutagenesis in cells offer advantages over in vitro mutagenesis, but current in vivo methods suffer from a lack of control, genomic instability, low efficiency and narrow mutational spectra. Using a mechanism-driven approach, we created a potent, inducible, broad-spectrum and vector-based mutagenesis system in E. coli that enhances mutation 322,000-fold over basal levels, surpassing the mutational efficiency and spectra of widely used in vivo and in vitro methods. We demonstrate that this system can be used to evolve antibiotic resistance in wild-type E. coli in

Year of Publication
2015
Journal
Nat Commun
Volume
6
Pages
8425
Date Published
2015 Oct 07
ISSN
2041-1723
DOI
10.1038/ncomms9425
PubMed ID
26443021
PubMed Central ID
PMC4633624
Links
Grant list
Howard Hughes Medical Institute / United States
R01 GM065400 / GM / NIGMS NIH HHS / United States