Antibodies to biotin enable large-scale detection of biotinylation sites on proteins.

Nat Methods
Authors
Keywords
Abstract

Although purification of biotinylated molecules is highly efficient, identifying specific sites of biotinylation remains challenging. We show that anti-biotin antibodies enable unprecedented enrichment of biotinylated peptides from complex peptide mixtures. Live-cell proximity labeling using APEX peroxidase followed by anti-biotin enrichment and mass spectrometry yielded over 1,600 biotinylation sites on hundreds of proteins, an increase of more than 30-fold in the number of biotinylation sites identified compared to streptavidin-based enrichment of proteins.

Year of Publication
2017
Journal
Nat Methods
Volume
14
Issue
12
Pages
1167-1170
Date Published
2017 Dec
ISSN
1548-7105
DOI
10.1038/nmeth.4465
PubMed ID
29039416
Links