|Publication Type||Journal Article|
|Year of Publication||2017|
|Authors||Udeshi, ND, Pedram, K, Svinkina, T, Fereshetian, S, Myers, SA, Aygun, O, Krug, K, Clauser, K, Ryan, D, Ast, T, Mootha, VK, Ting, AY, Carr, SA|
|Date Published||2017 Oct 16|
Although purification of biotinylated molecules is highly efficient, identifying specific sites of biotinylation remains challenging. We show that anti-biotin antibodies enable unprecedented enrichment of biotinylated peptides from complex peptide mixtures. Live-cell proximity labeling using APEX peroxidase followed by anti-biotin enrichment and mass spectrometry yielded over 1,600 biotinylation sites on hundreds of proteins, an increase of more than 30-fold in the number of biotinylation sites identified compared to streptavidin-based enrichment of proteins.
|Alternate Journal||Nat. Methods|