Genetic evaluation of candidate genes for the Mom1 modifier of intestinal neoplasia in mice.

Genetics
Authors
Keywords
Abstract

As genetic mapping of quantitative trait loci (QTL) becomes routine, the challenge is to identify the underlying genes. This paper develops rigorous genetic tests for evaluation of candidate genes for a QTL, involving determination of allelic status in inbred strains and fine-structure genetic mapping. For the Mom1 modifier of intestinal adenomas caused by ApcMin, these tests are used to evaluate two candidate genes: Pla2g2a, a secretory phospholipase, and Rap1GAP, a GTPase activating protein. Rap1GAP passes the first test but is excluded by a single fine-structure recombinant. Pla2g2a passes both tests and is a strong candidate for Mom1. Significantly, we also find that ApcMin-induced adenomas remain heterozygous for the Mom1 region, consistent with Mom1 acting outside the tumor lineage and encoding a secreted product.

Year of Publication
1996
Journal
Genetics
Volume
144
Issue
4
Pages
1777-85
Date Published
1996 Dec
ISSN
0016-6731
PubMed ID
8978063
PubMed Central ID
PMC1207727
Links
Grant list
CA-07175 / CA / NCI NIH HHS / United States
CA-23076 / CA / NCI NIH HHS / United States
CA-50585 / CA / NCI NIH HHS / United States