Spatial maps of T cell receptors and transcriptomes reveal distinct immune niches and interactions in the adaptive immune response.
Publication type | Journal Article
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Authors | |
Abstract | T cells mediate antigen-specific immune responses to disease through the specificity and diversity of their clonotypic T cell receptors (TCRs). Determining the spatial distributions of T cell clonotypes in tissues is essential to understanding T cell behavior, but spatial sequencing methods remain unable to profile the TCR repertoire. Here, we developed Slide-TCR-seq, a 10-μm-resolution method, to sequence whole transcriptomes and TCRs within intact tissues. We confirmed the ability of Slide-TCR-seq to map the characteristic locations of T cells and their receptors in mouse spleen. In human lymphoid germinal centers, we identified spatially distinct TCR repertoires. Profiling T cells in renal cell carcinoma and melanoma specimens revealed heterogeneous immune responses: T cell states and infiltration differed intra- and inter-clonally, and adjacent tumor and immune cells exhibited distinct gene expression. Altogether, our method yields insights into the spatial relationships between clonality, neighboring cell types, and gene expression that drive T cell responses. |
Year of Publication | 2022
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Journal | Immunity
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Volume | 55
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Issue | 10
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Pages | 1940-1952.e5
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Date Published | 2022 Oct 11
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ISSN | 1097-4180
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DOI | 10.1016/j.immuni.2022.09.002
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PubMed ID | 36223726
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