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Cell DOI:10.1016/j.cell.2016.08.057

Inhibition of Dihydroorotate Dehydrogenase Overcomes Differentiation Blockade in Acute Myeloid Leukemia.

Publication TypeJournal Article
Year of Publication2016
AuthorsSykes, DB, Kfoury, YS, Mercier, FE, Wawer, MJ, Law, JM, Haynes, MK, Lewis, TA, Schajnovitz, A, Jain, E, Lee, D, Meyer, H, Pierce, KA, Tolliday, NJ, Waller, A, Ferrara, SJ, Eheim, AL, Stoeckigt, D, Maxcy, KL, Cobert, JM, Bachand, J, Szekely, BA, Mukherjee, S, Sklar, LA, Kotz, JD, Clish, CB, Sadreyev, RI, Clemons, PA, Janzer, A, Schreiber, SL, Scadden, DT
JournalCell
Volume167
Issue1
Pages171-186.e15
Date Published2016 Sep 22
ISSN1097-4172
KeywordsAnimals, Antineoplastic Agents, Cell Differentiation, Enzyme Inhibitors, High-Throughput Screening Assays, Homeodomain Proteins, Humans, Leukemia, Myeloid, Acute, Mice, Molecular Targeted Therapy, Myeloid Cells, Oxidoreductases Acting on CH-CH Group Donors, Pyrimidines, Small Molecule Libraries, Xenograft Model Antitumor Assays
Abstract

While acute myeloid leukemia (AML) comprises many disparate genetic subtypes, one shared hallmark is the arrest of leukemic myeloblasts at an immature and self-renewing stage of development. Therapies that overcome differentiation arrest represent a powerful treatment strategy. We leveraged the observation that the majority of AML, despite their genetically heterogeneity, share in the expression of HoxA9, a gene normally downregulated during myeloid differentiation. Using a conditional HoxA9 model system, we performed a high-throughput phenotypic screen and defined compounds that overcame differentiation blockade. Target identification led to the unanticipated discovery that inhibition of the enzyme dihydroorotate dehydrogenase (DHODH) enables myeloid differentiation in human and mouse AML models. In vivo, DHODH inhibitors reduced leukemic cell burden, decreased levels of leukemia-initiating cells, and improved survival. These data demonstrate the role of DHODH as a metabolic regulator of differentiation and point to its inhibition as a strategy for overcoming differentiation blockade in AML.

DOI10.1016/j.cell.2016.08.057
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/27641501?dopt=Abstract

Alternate JournalCell
PubMed ID27641501
Grant ListS10 OD012027 / OD / NIH HHS / United States
R03 DA032471 / DA / NIDA NIH HHS / United States
U54 HG005032 / HG / NHGRI NIH HHS / United States
U54 MH084690 / MH / NIMH NIH HHS / United States