|Publication Type||Journal Article|
|Year of Publication||2022|
|Authors||Najjar, D, Rainbow, J, Timilsina, SSharma, Jolly, P, de Puig, H, Yafia, M, Durr, N, Sallum, H, Alter, G, Li, JZ, Yu, XG, Walt, DR, Paradiso, JA, Estrela, P, Collins, JJ, Ingber, DE|
|Journal||Nat Biomed Eng|
|Date Published||2022 Aug 08|
Rapid, accurate and frequent detection of the RNA of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) and of serological host antibodies to the virus would facilitate the determination of the immune status of individuals who have Coronavirus disease 2019 (COVID-19), were previously infected by the virus, or were vaccinated against the disease. Here we describe the development and application of a 3D-printed lab-on-a-chip that concurrently detects, via multiplexed electrochemical outputs and within 2 h, SARS-CoV-2 RNA in saliva as well as anti-SARS-CoV-2 immunoglobulins in saliva spiked with blood plasma. The device automatedly extracts, concentrates and amplifies SARS-CoV-2 RNA from unprocessed saliva, and integrates the Cas12a-based enzymatic detection of SARS-CoV-2 RNA via isothermal nucleic acid amplification with a sandwich-based enzyme-linked immunosorbent assay on electrodes functionalized with the Spike S1, nucleocapsid and receptor-binding-domain antigens of SARS-CoV-2. Inexpensive microfluidic electrochemical sensors for performing multiplexed diagnostics at the point of care may facilitate the widespread monitoring of COVID-19 infection and immunity.
|Alternate Journal||Nat Biomed Eng|
|PubMed Central ID||PMC9361916|