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Nat Biomed Eng DOI:10.1038/s41551-022-00919-w

A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma.

Publication TypeJournal Article
Year of Publication2022
AuthorsNajjar, D, Rainbow, J, Timilsina, SSharma, Jolly, P, de Puig, H, Yafia, M, Durr, N, Sallum, H, Alter, G, Li, JZ, Yu, XG, Walt, DR, Paradiso, JA, Estrela, P, Collins, JJ, Ingber, DE
JournalNat Biomed Eng
Date Published2022 Aug 08

Rapid, accurate and frequent detection of the RNA of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) and of serological host antibodies to the virus would facilitate the determination of the immune status of individuals who have Coronavirus disease 2019 (COVID-19), were previously infected by the virus, or were vaccinated against the disease. Here we describe the development and application of a 3D-printed lab-on-a-chip that concurrently detects, via multiplexed electrochemical outputs and within 2 h, SARS-CoV-2 RNA in saliva as well as anti-SARS-CoV-2 immunoglobulins in saliva spiked with blood plasma. The device automatedly extracts, concentrates and amplifies SARS-CoV-2 RNA from unprocessed saliva, and integrates the Cas12a-based enzymatic detection of SARS-CoV-2 RNA via isothermal nucleic acid amplification with a sandwich-based enzyme-linked immunosorbent assay on electrodes functionalized with the Spike S1, nucleocapsid and receptor-binding-domain antigens of SARS-CoV-2. Inexpensive microfluidic electrochemical sensors for performing multiplexed diagnostics at the point of care may facilitate the widespread monitoring of COVID-19 infection and immunity.


Alternate JournalNat Biomed Eng
PubMed ID35941191
PubMed Central IDPMC9361916