Single-cell analyses of Crohn's disease tissues reveal intestinal intraepithelial T cells heterogeneity and altered subset distributions.
Crohn's disease (CD) is a chronic transmural inflammation of intestinal segments caused by dysregulated interaction between microbiome and gut immune system. Here, we profile, via multiple single-cell technologies, T cells purified from the intestinal epithelium and lamina propria (LP) from terminal ileum resections of adult severe CD cases. We find that intraepithelial lymphocytes (IEL) contain several unique T cell subsets, including NKp30γδT cells expressing RORγt and producing IL-26 upon NKp30 engagement. Further analyses comparing tissues from non-inflamed and inflamed regions of patients with CD versus healthy controls show increased activated T17 but decreased CD8T, γδT, T and Treg cells in inflamed tissues. Similar analyses of LP find increased CD8, as well as reduced CD4T cells with an elevated T17 over Treg/T ratio. Our analyses of CD tissues thus suggest a potential link, pending additional validations, between transmural inflammation, reduced IEL γδT cells and altered spatial distribution of IEL and LP T cell subsets.
|Year of Publication
2021 03 26
|PubMed Central ID
R01 DK124699 / DK / NIDDK NIH HHS / United States
U19 AI142733 / AI / NIAID NIH HHS / United States
P30 DK043351 / DK / NIDDK NIH HHS / United States
P30 DK052574 / DK / NIDDK NIH HHS / United States
UL1 TR002345 / TR / NCATS NIH HHS / United States