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Cell Host Microbe DOI:10.1016/j.chom.2016.07.006

Fap2 Mediates Fusobacterium nucleatum Colorectal Adenocarcinoma Enrichment by Binding to Tumor-Expressed Gal-GalNAc.

Publication TypeJournal Article
Year of Publication2016
AuthorsAbed, J, Emgård, JEM, Zamir, G, Faroja, M, Almogy, G, Grenov, A, Sol, A, Naor, R, Pikarsky, E, Atlan, KA, Mellul, A, Chaushu, S, Manson, AL, Earl, AM, Ou, N, Brennan, CA, Garrett, WS, Bachrach, G
JournalCell Host Microbe
Volume20
Issue2
Pages215-25
Date Published2016 Aug 10
ISSN1934-6069
Abstract

Fusobacterium nucleatum is associated with colorectal cancer and promotes colonic tumor formation in preclinical models. However, fusobacteria are core members of the human oral microbiome and less prevalent in the healthy gut, raising questions about how fusobacteria localize to CRC. We identify a host polysaccharide and fusobacterial lectin that explicates fusobacteria abundance in CRC. Gal-GalNAc, which is overexpressed in CRC, is recognized by fusobacterial Fap2, which functions as a Gal-GalNAc lectin. F. nucleatum binding to clinical adenocarcinomas correlates with Gal-GalNAc expression and is reduced upon O-glycanase treatment. Clinical fusobacteria strains naturally lacking Fap2 or inactivated Fap2 mutants show reduced binding to Gal-GalNAc-expressing CRC cells and established CRCs in mice. Additionally, intravenously injected F. nucleatum localizes to mouse tumor tissues in a Fap2-dependent manner, suggesting that fusobacteria use a hematogenous route to reach colon adenocarcinomas. Thus, targeting F. nucleatum Fap2 or host epithelial Gal-GalNAc may reduce fusobacteria potentiation of CRC.

DOI10.1016/j.chom.2016.07.006
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/27512904?dopt=Abstract

Alternate JournalCell Host Microbe
PubMed ID27512904
Grant ListU19 AI110818 / AI / NIAID NIH HHS / United States