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Commun Biol DOI:10.1038/s42003-021-02276-x

Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging.

Publication TypeJournal Article
Year of Publication2021
AuthorsTomov, ML, O'Neil, A, Abbasi, HS, Cimini, BA, Carpenter, AE, Rubin, LL, Bathe, M
JournalCommun Biol
Date Published2021 06 24
KeywordsAnimals, Cell Differentiation, Cells, Cultured, Cerebral Cortex, Humans, Induced Pluripotent Stem Cells, Motor Neurons, Optical Imaging, Rats

Human induced pluripotent stem cell-derived (iPSC) neural cultures offer clinically relevant models of human diseases, including Amyotrophic Lateral Sclerosis, Alzheimer's, and Autism Spectrum Disorder. In situ characterization of the spatial-temporal evolution of cell state in 3D culture and subsequent 2D dissociated culture models based on protein expression levels and localizations is essential to understanding neural cell differentiation, disease state phenotypes, and sample-to-sample variability. Here, we apply PRobe-based Imaging for Sequential Multiplexing (PRISM) to facilitate multiplexed imaging with facile, rapid exchange of imaging probes to analyze iPSC-derived cortical and motor neuron cultures that are relevant to psychiatric and neurodegenerative disease models, using over ten protein targets. Our approach permits analysis of cell differentiation, cell composition, and functional marker expression in complex stem-cell derived neural cultures. Furthermore, our approach is amenable to automation, offering in principle the ability to scale-up to dozens of protein targets and samples.


Alternate JournalCommun Biol
PubMed ID34168275
PubMed Central IDPMC8225800
Grant ListP30 ES002109 / ES / NIEHS NIH HHS / United States
R01 MH112694 / MH / NIMH NIH HHS / United States
U01 MH106011 / MH / NIMH NIH HHS / United States