Genetic Perturbation Program

A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.

Arroyo JD, Jourdain AA, Calvo SE, et al. A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation. Cell Metab. 2016;24(6):875-885. doi:10.1016/j.cmet.2016.08.017DOI PubMed Google Scholar

Bromodomain Inhibitors Correct Bioenergetic Deficiency Caused by Mitochondrial Disease Complex I Mutations.

Barrow JJ, Balsa E, Verdeguer F, et al. Bromodomain Inhibitors Correct Bioenergetic Deficiency Caused by Mitochondrial Disease Complex I Mutations. Mol Cell. 2016;64(1):163-175. doi:10.1016/j.molcel.2016.08.023DOI PubMed Google Scholar

Discovery of a proteinaceous cellular receptor for a norovirus.

Orchard RC, Wilen CB, Doench JG, et al. Discovery of a proteinaceous cellular receptor for a norovirus. Science. 2016;353(6302):933-6. doi:10.1126/science.aaf1220DOI PubMed Google Scholar

CRISPR/Cas9 Screens Reveal Requirements for Host Cell Sulfation and Fucosylation in Bacterial Type III Secretion System-Mediated Cytotoxicity.

Blondel CJ, Park JS, Hubbard TP, et al. CRISPR/Cas9 Screens Reveal Requirements for Host Cell Sulfation and Fucosylation in Bacterial Type III Secretion System-Mediated Cytotoxicity. Cell Host Microbe. 2016;20(2):226-37. doi:10.1016/j.chom.2016.06.010DOI PubMed Google Scholar

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9.

Doench JG, Fusi N, Sullender M, et al. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016;34(2):184-91. doi:10.1038/nbt.3437DOI PubMed Google Scholar

Genome-scale CRISPR-Cas9 knockout screening in human cells.

Shalem O, Sanjana NE, Hartenian E, et al. Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014;343(6166):84-7. doi:10.1126/science.1247005DOI PubMed Google Scholar