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Methods for Determining Spatial and Temporal Gene Expression Dynamics During Adult Neurogenesis in Single Cells

Application No.

PCT/US2018/060860

Broad Case No.

BI-10283

List of inventors -

Inbal Avraham-Davidi, Anindita Basu, Eugene Drokhlyansky, Naomi Habib, Aviv Regev, Orit Rozenblatt-Rosen, David Weitz

Abstract

Techniques Nuc-seq, Div-Seq, and Dronc-Seq are allow for unbiased analysis of any complex tissue. Nuc-Seq, a scalable single nucleus RNA-Seq method, can sensitively identify closely related cell types, including within the adult hippocampus. Div-seq combines Nuc-Seq with EdU-mediated labeling of proliferating cells, allowing tracking of transcriptional dynamics of newborn neurons in an adult neurogenic region in the hippocampus. Dronc-Seq uses a microfluidic device to co-encapsulate individual nuclei in reverse emulsion aqueous droplets in an oil medium together with one uniquely barcoded mRNA-capture bead.

Date PUBLISHED

  • 05/16/2019

Published Patent Application

  • WO2019/094984

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