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Methods for Designing Guides Sequences for Guided Nucleases

Application No.

PCT/US2018/041041

Broad Case No.

BI-10209

List of inventors -

Joshua Meier, Neville Sanjana, Feng Zhang

Abstract

Embodiments disclosed herein provide methods, including computer-implemented methods, for designing guide sequence which may be incorporated into custom, large scale guide sequence libraries. The methods require only a list of target genes as input and utilize on target and off target scores to generate an optimal set of guide sequences for a set of target genes. In certain embodiments, the methods may also utilize multi-tissue RNA-sequencing data and/or protein annotation to design targets to genes that are highly expressed and/or contain a functional protein domain. The invention further comprises guide libraries, cells comprising said guide libraries. Computer-implemented embodiments further improve computer system function by reducing excessive user wait time through the use of data structures that reduce search from linear to logarithmic time.

Date PUBLISHED

  • 01/10/2019

Published Patent Application

  • WO2019/010384

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