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Functional genomics using crispr-cas systems for saturating mutagenesis of non-coding elements, compositions, methods, libraries and applications thereof

Application No.

PCT/US2016/031164

Broad Case No.

BI-2015/098

List of inventors -

Feng Zhang, Neville Sanjana, Stuart Orkin, Ophir Shalem, Jason Wright, Daniel Bauer

Abstract

The application relates to a deep scanning mutagenesis library to interrogate phenotypi c changes in a population of cells comprising a plurality of CRISPR-Cas system guide RNAs targeting genomic sequences within at least one continuous genomic region, wherein the guide RNAs target at least 100 genomic sequences upstream of a PAM sequence for every 1000 base pairs within the continuous genomic region and methods for their use.

Date PUBLISHED

  • 11/17/2016

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