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Double Selection HDR CRISPR-Based Editing

Application No.

PCT/US2018/048943

Broad Case No.

BI-10163

List of inventors -

Melina Claussnitzer, Sarah Goggin, Alham Saadat

Abstract

The invention provides homology directed repair (HDR) constructs for variant screening in cells comprising: a left and right homology arm, with either the left or right homology arm encoding a genomic edit to be incorporated at a target locus; and an excisable double selection cassette located within the left and right homology arms, the excisable double selection cassette comprising; a first selection marker; and a second selection marker; and wherein the first selection marker and the second selection marker are located between a first and second excision site. Also provided are homology directed repair (HDR) vectors comprising a construct as described herein, and methods for using such vectors.

Date PUBLISHED

  • 03/07/2019

Published Patent Application

  • WO2019/046636

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