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Constructs for continuous monitoring of live cells

Application No.

PCT/US2016/022722

Broad Case No.

BI-2014/077

List of inventors -

Paul Blainey, Jacob Borrajo

Abstract

Embodiments disclosed herein provide nucleic acid constructs and methods of use thereof that induce a live cell to give off sub-samples of the cell's cytosolic content. The term "cell" as used herein may be any cell type. In certain example embodiments, the cells are mammalian cells. The sampling can be general or can be targeted to a particular class of molecules or to specific types of molecules. The constructs facilitate generation of a read-out for high-throughput screens by combining engineered export with simple bulk sample and sample processing. Live cell sampling enables time course measurements and expands, for example, the applicability of transcriptional profiles obtained by single cell gene expression analysis.
 


 

Date PUBLISHED

  • 09/22/2016

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