BroadE: CRISPR in a nutshell

This fall 2017 BroadE workshop covered:

1. Introduction to CRISPR-Cas systems and basic genome editing methodologies (focusing on Cas9), including guide design, in vitro and in vivo delivery, and assaying results
2. Guide to CRISPR-Cas9-based screening applications (both loss- and gain-of-function)
3. Overview of frontiers in genome editing, including therapeutic delivery modes, approaches to enhancing specificity, and novel RNA-guided RNA-targeting enzymes