New technology puts a spatial lens on CRISPR screening
Perturb-FISH reveals impacts of perturbations on gene expression and phenotype with single-cell, spatial resolution, allowing study of effects within and between cells.

Credit: Broad Communications
Scientists in the Spatial Technology Platform at the Broad develop, use, and share spatial -omics approaches, such as Perturb-FISH, that can reveal the roles of genes in various cell types and the impact of intercellular interactions on health and disease.
Funding
Funding statement: This work was supported in part by the National Institutes of Health, the Israel Science Foundation, and the Simons Foundation Autism Research Initiative.
Paper cited
Binan L, et al. Simultaneous CRISPR screening and spatial transcriptomics reveals intracellular, intercellular, and functional transcriptional circuits. Cell. Online March 12, 2025.