MitoCarta3.0: An Inventory of Mammalian Mitochondrial Proteins and Pathways

MitoCarta3.0 is an inventory of 1136 human and 1140 mouse genes encoding proteins with strong support of mitochondrial localization, now with sub-mitochondrial compartment and pathway annotations. To generate this inventory, we performed mass spectrometry of mitochondria isolated from fourteen tissues, assessed protein localization through large-scale GFP tagging/microscopy, and integrated these results with six other genome-scale datasets of mitochondrial localization, using a Bayesian approach. MitoCarta3.0, released 2020, uses manual literature curation to revise the previous MitoCarta2.0 inventory (78 added and 100 removed genes), provide annotation of sub-mitochondrial localization, and assign genes to a custom ontology of 149 mitochondrial pathways. 

 

View MitoCarta3.0 datasets
  Human MitoCarta3.0  1136 MitoCarta genes: evidence of mitochondrial protein localization and protein distribution across 14 tissues
Mouse MitoCarta3.0 1140 MitoCarta genes: evidence of mitochondrial protein localization and protein distribution across 14 tissues
MitoPathways3.0 MitoCarta3.0 genes manually curated into 149 mitochondrial pathways

 

Download MitoCarta3.0 datasets
  Human.MitoCarta3.0.xls Excel database containing detailed information on the 1136 mitochondrial human genes including sub-mitochondrial localization and pathways, as well as integrated scores of mitochondrial localization for all ~20,000 human genes
  Mouse.MitoCarta3.0.xls Excel database containing detailed information on the 1140 mitochondrial mouse genes including sub-mitochondrial localization and pathways, as well as integrated scores of mitochondrial localization for all ~20,000 mouse genes
  MitoPathways3.0.gmx GMX format file of genes assigned to 149 mitochondrial pathways, useful for GSEA tool
  Human.MitoCarta3.0.bed BED file of human MitoCarta3.0 genes within UCSC assembly hg19. Use UCSC's Add Custom Tracks to add URL http://www.broadinstitute.org/ftp/ distribution/metabolic/
papers/Pagliarini/MitoCarta3.0/Human.MitoCarta3.0.bed
  Mouse.MitoCarta3.0.bed BED file of mouse MitoCarta3.0 genes within UCSC assembly mm10. Use UCSC's Add Custom Tracks to add URL http://www.broadinstitute.org/ftp/ distribution/metabolic/
papers/Pagliarini/MitoCarta3.0/Mouse.MitoCarta3.0.bed
  Human.MitoCarta3.0.fasta Fasta file of human MitoCarta3.0 RefSeq protein sequences
  Mouse.MitoCarta3.0.fasta Fasta file of mouse MitoCarta3.0 RefSeq protein sequences
  GFP localization Images of 131 protein with mitochondrial localization assessed by GFP tagging and microscopy
  Mouse.MSMS.peptides.xlsx Excel file of 92366 unique MS/MS peptides detected in 14 mouse tissues

 

Previous versions

 

Citation:
  • Rath, S.,* Sharma, R.*, Gupta, R.*, ..., Calvo, S.E., Mootha, V.K.. MitoCarta3.0: An updated inventory of the mitochondrial proteome, now with sub-organelle localization and pathway annotations (2020). Submitted
  • Calvo, S.E., Klauser, C.R., Mootha, V.K. MitoCarta2.0: an updated inventory of mammalian mitochondrial proteins (2015).  Nucleic Acids Research [doi: 10.1093/nar/gkv1003]
  • Pagliarini, D.J., Calvo, S.E., Chang, B., Sheth, S.A., Vafai, S.B., Ong, S.E., Walford, G.A., Sugiana, C., Boneh, A., Chen, W.K., et al. (2008). A mitochondrial protein compendium elucidates complex I disease biology. Cell 134, 112-123. [Pubmed: 18614015]