MitoCarta3.0 is an inventory of 1136 human and 1140 mouse genes encoding proteins with strong support of mitochondrial localization, now with sub-mitochondrial compartment and pathway annotations. To generate this inventory, we performed mass spectrometry of mitochondria isolated from fourteen tissues, assessed protein localization through large-scale GFP tagging/microscopy, and integrated these results with six other genome-scale datasets of mitochondrial localization, using a Bayesian approach. MitoCarta3.0, released 2020, uses manual literature curation to revise the previous MitoCarta2.0 inventory (78 added and 100 removed genes), provide annotation of sub-mitochondrial localization, and assign genes to a custom ontology of 149 mitochondrial pathways.
View MitoCarta3.0 datasets
| Human MitoCarta3.0 | 1136 MitoCarta genes: evidence of mitochondrial protein localization and protein distribution across 14 tissues | |
| Mouse MitoCarta3.0 | 1140 MitoCarta genes: evidence of mitochondrial protein localization and protein distribution across 14 tissues | |
| MitoPathways3.0 | MitoCarta3.0 genes manually curated into 149 mitochondrial pathways |
Download MitoCarta3.0 datasets (Chrome browser instructions below)
| Human.MitoCarta3.0.xls | Excel database containing detailed information on the 1136 mitochondrial human genes including sub-mitochondrial localization and pathways, as well as integrated scores of mitochondrial localization for all ~20,000 human genes | |
| Mouse.MitoCarta3.0.xls | Excel database containing detailed information on the 1140 mitochondrial mouse genes including sub-mitochondrial localization and pathways, as well as integrated scores of mitochondrial localization for all ~20,000 mouse genes | |
| MitoPathways3.0.gmx | GMX format file of genes assigned to 149 mitochondrial pathways, useful for GSEA tool | |
| Human.MitoCarta3.0.bed |
BED file of human MitoCarta3.0 genes within UCSC assembly hg19. Use UCSC's Add Custom Tracks to add URL http://www.broadinstitute.org/ftp/ distribution/metabolic/ papers/Pagliarini/MitoCarta3.0/ |
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| Mouse.MitoCarta3.0.bed |
BED file of mouse MitoCarta3.0 genes within UCSC assembly mm10. Use UCSC's Add Custom Tracks to add URL http://www.broadinstitute.org/ftp/ distribution/metabolic/ papers/Pagliarini/MitoCarta3.0/ |
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| Human.MitoCarta3.0.fasta | Fasta file of human MitoCarta3.0 RefSeq protein sequences | |
| Mouse.MitoCarta3.0.fasta | Fasta file of mouse MitoCarta3.0 RefSeq protein sequences | |
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Images of 131 protein with mitochondrial localization assessed by GFP tagging and microscopy | |
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Excel file of 92366 unique MS/MS peptides detected in 14 mouse tissues |
Previous versions
Citation:
- Rath, S.,* Sharma, R.*, Gupta, R.*, ..., Calvo, S.E., Mootha, V.K.. MitoCarta3.0: An updated inventory of the mitochondrial proteome, now with sub-organelle localization and pathway annotations (2020). Nucleic Acids Research [Pubmed: 33174596]
- Calvo, S.E., Klauser, C.R., Mootha, V.K. MitoCarta2.0: an updated inventory of mammalian mitochondrial proteins (2015). Nucleic Acids Research [doi: 10.1093/nar/gkv1003]
- Pagliarini, D.J., Calvo, S.E., Chang, B., Sheth, S.A., Vafai, S.B., Ong, S.E., Walford, G.A., Sugiana, C., Boneh, A., Chen, W.K., et al. (2008). A mitochondrial protein compendium elucidates complex I disease biology. Cell 134, 112-123. [Pubmed: 18614015]
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