MitoCarta2.0 Documentation

GeneID NCBI Entrez gene identifier
Symbol Entrez official gene symbol
Description Entrez gene description
Synonyms Entrez gene synonyms
Maestro Score Integrated score of mitochondrial localization. Score of 5.22 (human) or 5.30 (mouse) indicates 5% cFDR cutoff, but genes with literature or GFP support may have lower scores.
FDR Estimated false discovery rate associated with all genes with this Maestro score or highter, estimated from large training datasets
Evidence Evidence of mitochondrial localization.
Note: a data source is listed as evidence of mitochondrial localization if LogOdds>1 (based on training data); "+" suffix indicates strong support (LogOdds>5), and "++" suffix indicates very strong support (LogOdds>7).
  • literature: manually curated list of genes encoding proteins with strong support of mitochondrial localization in mammals from literature (not including previous large-scale proteomic studies)
  • APEX_matrix: detected in the mitochondrial matrix in HEK293 cells based on APEX labeling [Rhee et al., Science 2013 PMID:23371551]
  • APEX_IMS: detected in the mitochondrial intermembrane space in HEK293 cells based on APEX labeling [Hung et al., Mol Cell 2014 PMID:25002142]
  • GFP: clear mitochondrial localization observed by GFP-tagging and microscopy in our study
  • targetP signal: mitochondrial targeting signal present in N-terminal 130 aa, based on targetP
  • yeast mito homolog: best S. cerevisiase homolog, based on BlastP, is a curated mitochondrial gene
  • Rickettsial homolog: gene has a homolog to R. prowazekii based on BlastP (with expect < 1e-3) or jackHMMER reciprocal hit
  • mito protein domain: protein has a mitochondrial-specific Pfam domain (++ suffix), or Pfam domain present in both mitochondrial and non-mitochondrial proteins (no suffix)
  • induction: mouse transcript was induced during mitochondrial biogenesis
  • coexpression: mouse transcript is coexpressed with known mitochondrial genes across mouse tissues (+ and ++ indicate higher levels of coexpression)
  • MS/MS: mouse protein detected with high confidence in mitochondrial samples from 14 tissues (+ and ++ indicate higher confidence of detection)
Tissues Protein detected by discovery MS/MS within 14 mouse tissues analyzed:
1: cerebrum
2: cerebellem
3: brainstem
4: spinal cord
5: liver
6: kidney
7: heart
8: skeletal muscle
9: adipose
10: stomach
11: sm intestine
12: lg intestine
13: testis
14: placenta