Protein–protein interactions are central to biological function, making their design highly relevant for therapeutic and biotechnological applications. BindCraft is a user-friendly, open-source pipeline that enables high-accuracy binder hallucination based on AlphaFold2.
In this workshop, participants will learn the theoretical foundations of binder design, how to identify binding sites and interaction hotspots, prepare input structures and configuration files, and run a standard BindCraft workflow. They will also gain insights into writing and customizing loss functions to guide design objectives, along with practical ideas for the experimental validation of designed binders.