# Tagged with #leftalignvariants 1 documentation article | 0 announcements | 2 forum discussions

Created 2012-07-23 23:56:00 | Updated 2012-07-23 23:56:00 | Tags: leftalignvariants gatkdocs

A new tool has been released!

Check out the documentation at LeftAlignVariants.

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Created 2013-01-05 00:05:51 | Updated | Tags: leftalignvariants

We have a complex VCF record that doesn't appear to be properly treated by LeftAlignVariants, and I couldn't find evidence that this behavior has been reported anywhere else.

The record is

17      19561175        .       GGTTTGT G,GTTTGT        49      PASS    AC=1,1;AF=0.50,0.50;AN=2;DP=117;DS;MQ=60;MQ0=0;source=Locus     GT:AB:AD:DP     1/2:0.925:68,49:117


Admittedly, the GGTTTGT>GTTTGT variation is odd because it's better specified as GG>G, but there's nothing semantically wrong with this record as written. (if you're wondering where this came from, it came from simulated data)

The challenge however is that the left-aligned version of this variant is AG>G. So I could see expecting the following output from LeftAlignVariants:

17      19561174        .       AGGTTTGT AG,AGTTTGT


Rather ugly, but I think that's the right way to write the original complex variation after left-alignment. Alternatively a separated and phased representation would achieve the same:

17      19561174        .       AG  A             .... 0/1:...
17      19561175        .       GGTTTGT G   .... 1|0:...


But I bet that would introduce all types of problems in LeftAlignVariants if you tried to make that happen.

I think it's a really hard problem to solve in the main, just wanted to post here to see if 1. You agree that it behaves this way and 2. Help anyone else who might be seeing something like this.

Created 2012-07-24 14:17:40 | Updated 2012-07-24 14:19:56 | Tags: indelrealigner leftalignindels leftalignvariants

Dear all,

I was browsing through some of the less used functions in the GATK documentation, hence the following question: Does the LeftAlignIndels function do something additional that is not happening with IndelRealigner? In other words, do you recommend to run LeftAlignIndels on top of the indel realignment?

Best regards, Sophia