Detailed information about command line options for BaseRecalibrator can be found here.
The tools in this package recalibrate base quality scores of sequencing-by-synthesis reads in an aligned BAM file. After recalibration, the quality scores in the QUAL field in each read in the output BAM are more accurate in that the reported quality score is closer to its actual probability of mismatching the reference genome. Moreover, the recalibration tool attempts to correct for variation in quality with machine cycle and sequence context, and by doing so provides not only more accurate quality scores but also more widely dispersed ones. The system works on BAM files coming from many sequencing platforms: Illumina, SOLiD, 454, Complete Genomics, Pacific Biosciences, etc.
New with the release of the full version of GATK 2.0 is the ability to recalibrate not only the well-known base quality scores but also base insertion and base deletion quality scores. These are per-base quantities which estimate the probability that the next base in the read was mis-incorporated or mis-deleted (due to slippage, for example). We've found that these new quality scores are very valuable in indel calling algorithms. In particular these new probabilities fit very naturally as the gap penalties in an HMM-based indel calling algorithms. We suspect there are many other fantastic uses for these data.
This process is accomplished by analyzing the covariation among several features of a base. For example:
These covariates are then subsequently applied through a piecewise tabular correction to recalibrate the quality scores of all reads in a BAM file.
For example, pre-calibration a file could contain only reported Q25 bases, which seems good. However, it may be that these bases actually mismatch the reference at a 1 in 100 rate, so are actually Q20. These higher-than-empirical quality scores provide false confidence in the base calls. Moreover, as is common with sequencing-by-synthesis machine, base mismatches with the reference occur at the end of the reads more frequently than at the beginning. Also, mismatches are strongly associated with sequencing context, in that the dinucleotide AC is often much lower quality than TG. The recalibration tool will not only correct the average Q inaccuracy (shifting from Q25 to Q20) but identify subsets of high-quality bases by separating the low-quality end of read bases AC bases from the high-quality TG bases at the start of the read. See below for examples of pre and post corrected values.
The system was designed for users to be able to easily add new covariates to the calculations. For users wishing to add their own covariate simply look at QualityScoreCovariate.java for an idea of how to implement the required interface. Each covariate is a Java class which implements the org.broadinstitute.sting.gatk.walkers.recalibration.Covariate interface. Specifically, the class needs to have a getValue method defined which looks at the read and associated sequence context and pulls out the desired information such as machine cycle.
Detailed information about command line options for BaseRecalibrator can be found here.
This GATK processing step walks over all of the reads in
my_reads.bam and tabulates data about the following features of the bases:
For each bin, we count the number of bases within the bin and how often such bases mismatch the reference base, excluding loci known to vary in the population, according to dbSNP. After running over all reads, BaseRecalibrator produces a file called
my_reads.recal_data.grp, which contains the data needed to recalibrate reads. The format of this GATK report is described below.
To create a recalibrated BAM you can use GATK's PrintReads with the engine on-the-fly recalibration capability. Here is a typical command line to do so:
java -jar GenomeAnalysisTK.jar \ -T PrintReads \ -R reference.fasta \ -I input.bam \ -BQSR recalibration_report.grp \ -o output.bam
After computing covariates in the initial BAM File, we then walk through the BAM file again and rewrite the quality scores (in the QUAL field) using the data in the
recalibration_report.grp file, into a new BAM file.
This step uses the recalibration table data in recalibration_report.grp produced by BaseRecalibration to recalibrate the quality scores in input.bam, and writing out a new BAM file output.bam with recalibrated QUAL field values.
Effectively the new quality score is:
Following recalibration, the read quality scores are much closer to their empirical scores than before. This means they can be used in a statistically robust manner for downstream processing, such as SNP calling. In additional, by accounting for quality changes by cycle and sequence context, we can identify truly high quality bases in the reads, often finding a subset of bases that are Q30 even when no bases were originally labeled as such.
Note that the BasRecalibrator no longer produces plots; this is now done by the AnalyzeCovariates tool.
The recalibration report is a [GATKReport](http://gatk.vanillaforums.com/discussion/1244/what-is-a-gatkreport) and not only contains the main result of the analysis, but it is also used as an input to all subsequent analyses on the data. The recalibration report contains the following 5 tables:
This is the table that contains all the arguments used to run BQSRv2 for this dataset. This is important for the on-the-fly recalibration step to use the same parameters used in the recalibration step (context sizes, covariates, ...).
Example Arguments table:
#:GATKTable:true:1:17::; #:GATKTable:Arguments:Recalibration argument collection values used in this run Argument Value covariate null default_platform null deletions_context_size 6 force_platform null insertions_context_size 6 ...
The GATK offers native support to quantize base qualities. The GATK quantization procedure uses a statistical approach to determine the best binning system that minimizes the error introduced by amalgamating the different qualities present in the specific dataset. When running BQSRv2, a table with the base counts for each base quality is generated and a 'default' quantization table is generated. This table is a required parameter for any other tool in the GATK if you want to quantize your quality scores.
The default behavior (currently) is to use no quantization when performing on-the-fly recalibration. You can override this by using the engine argument -qq. With -qq 0 you don't quantize qualities, or -qq N you recalculate the quantization bins using N bins on the fly. Note that quantization is completely experimental now and we do not recommend using it unless you are a super advanced user.
Example Arguments table:
#:GATKTable:true:2:94:::; #:GATKTable:Quantized:Quality quantization map QualityScore Count QuantizedScore 0 252 0 1 15972 1 2 553525 2 3 2190142 9 4 5369681 9 9 83645762 9 ...
This table contains the empirical quality scores for each read group, for mismatches insertions and deletions. This is not different from the table used in the old table recalibration walker.
#:GATKTable:false:6:18:%s:%s:%.4f:%.4f:%d:%d:; #:GATKTable:RecalTable0: ReadGroup EventType EmpiricalQuality EstimatedQReported Observations Errors SRR032768 D 40.7476 45.0000 2642683174 222475 SRR032766 D 40.9072 45.0000 2630282426 213441 SRR032764 D 40.5931 45.0000 2919572148 254687 SRR032769 D 40.7448 45.0000 2850110574 240094 SRR032767 D 40.6820 45.0000 2820040026 241020 SRR032765 D 40.9034 45.0000 2441035052 198258 SRR032766 M 23.2573 23.7733 2630282426 12424434 SRR032768 M 23.0281 23.5366 2642683174 13159514 SRR032769 M 23.2608 23.6920 2850110574 13451898 SRR032764 M 23.2302 23.6039 2919572148 13877177 SRR032765 M 23.0271 23.5527 2441035052 12158144 SRR032767 M 23.1195 23.5852 2820040026 13750197 SRR032766 I 41.7198 45.0000 2630282426 177017 SRR032768 I 41.5682 45.0000 2642683174 184172 SRR032769 I 41.5828 45.0000 2850110574 197959 SRR032764 I 41.2958 45.0000 2919572148 216637 SRR032765 I 41.5546 45.0000 2441035052 170651 SRR032767 I 41.5192 45.0000 2820040026 198762
This table contains the empirical quality scores for each read group and original quality score, for mismatches insertions and deletions. This is not different from the table used in the old table recalibration walker.
#:GATKTable:false:6:274:%s:%s:%s:%.4f:%d:%d:; #:GATKTable:RecalTable1: ReadGroup QualityScore EventType EmpiricalQuality Observations Errors SRR032767 49 M 33.7794 9549 3 SRR032769 49 M 36.9975 5008 0 SRR032764 49 M 39.2490 8411 0 SRR032766 18 M 17.7397 16330200 274803 SRR032768 18 M 17.7922 17707920 294405 SRR032764 45 I 41.2958 2919572148 216637 SRR032765 6 M 6.0600 3401801 842765 SRR032769 45 I 41.5828 2850110574 197959 SRR032764 6 M 6.0751 4220451 1041946 SRR032767 45 I 41.5192 2820040026 198762 SRR032769 6 M 6.3481 5045533 1169748 SRR032768 16 M 15.7681 12427549 329283 SRR032766 16 M 15.8173 11799056 309110 SRR032764 16 M 15.9033 13017244 334343 SRR032769 16 M 15.8042 13817386 363078 ...
This table has the empirical qualities for each covariate used in the dataset. The default covariates are cycle and context. In the current implementation, context is of a fixed size (default 6). Each context and each cycle will have an entry on this table stratified by read group and original quality score.
#:GATKTable:false:8:1003738:%s:%s:%s:%s:%s:%.4f:%d:%d:; #:GATKTable:RecalTable2: ReadGroup QualityScore CovariateValue CovariateName EventType EmpiricalQuality Observations Errors SRR032767 16 TACGGA Context M 14.2139 817 30 SRR032766 16 AACGGA Context M 14.9938 1420 44 SRR032765 16 TACGGA Context M 15.5145 711 19 SRR032768 16 AACGGA Context M 15.0133 1585 49 SRR032764 16 TACGGA Context M 14.5393 710 24 SRR032766 16 GACGGA Context M 17.9746 1379 21 SRR032768 45 CACCTC Context I 40.7907 575849 47 SRR032764 45 TACCTC Context I 43.8286 507088 20 SRR032769 45 TACGGC Context D 38.7536 37525 4 SRR032768 45 GACCTC Context I 46.0724 445275 10 SRR032766 45 CACCTC Context I 41.0696 575664 44 SRR032769 45 TACCTC Context I 43.4821 490491 21 SRR032766 45 CACGGC Context D 45.1471 65424 1 SRR032768 45 GACGGC Context D 45.3980 34657 0 SRR032767 45 TACGGC Context D 42.7663 37814 1 SRR032767 16 AACGGA Context M 15.9371 1647 41 SRR032764 16 GACGGA Context M 18.2642 1273 18 SRR032769 16 CACGGA Context M 13.0801 1442 70 SRR032765 16 GACGGA Context M 15.9934 1271 31 ...
The memory requirements of the recalibrator will vary based on the type of JVM running the application and the number of read groups in the input bam file.
If the application reports 'java.lang.OutOfMemoryError: Java heap space', increase the max heap size provided to the JVM by adding ' -Xmx????m' to the jvm_args variable in RecalQual.py, where '????' is the maximum available memory on the processing computer.
I've tried recalibrating my data using a downloaded file, such as NA12878 on 454, and apply the table to any of the chromosome BAM files always fails due to hitting my memory limit. I've tried giving it as much as 15GB but that still isn't enough.
All of our big merged files for 454 are running with -Xmx16000m arguments to the JVM -- it's enough to process all of the files. 32GB might make the 454 runs a lot faster though.
I have a recalibration file calculated over the entire genome (such as for the 1000 genomes trio) but I split my file into pieces (such as by chromosome). Can the recalibration tables safely be applied to the per chromosome BAM files?
Yes they can. The original tables needed to be calculated over the whole genome but they can be applied to each piece of the data set independently.
I'm working on a genome that doesn't really have a good SNP database yet. I'm wondering if it still makes sense to run base quality score recalibration without known SNPs.
The base quality score recalibrator treats every reference mismatch as indicative of machine error. True polymorphisms are legitimate mismatches to the reference and shouldn't be counted against the quality of a base. We use a database of known polymorphisms to skip over most polymorphic sites. Unfortunately without this information the data becomes almost completely unusable since the quality of the bases will be inferred to be much much lower than it actually is as a result of the reference-mismatching SNP sites.
However, all is not lost if you are willing to experiment a bit. You can bootstrap a database of known SNPs. Here's how it works:
For users concerned about run time please note this small analysis below showing the approximate number of reads per read group that are required to achieve a given level of recalibration performance. The analysis was performed with 51 base pair Illumina reads on pilot data from the 1000 Genomes Project. Downsampling can be achieved by specifying a genome interval using the -L option. For users concerned only with recalibration accuracy please disregard this plot and continue to use all available data when generating the recalibration table.