ChIP-seq

Input Requirement(s)
0.5-1 million cells per epitope, cross-linked according to our protocol

Batch Size(s): 24 samples (single epitope screen) or 96 samples (single epitope screen)

Description
Chromatin Immunoprecipitation followed by DNA sequencing (ChIP-seq) maps localization of DNA-binding or DNA-associated proteins and is frequently utilized to study genome-wide dynamics of protein-DNA interaction networks. We have developed an automated process that provides reproducible, high-throughput and cost-effective ChIP and library construction, coupled to multiplexed sequencing (Garber et. al., 2012). Our standard ChIP-seq service currently operates with in-house-qualified monoclonal antibodies targeting RNAPolII, the chromatin regulator Sirt6, the bromodomain protein Brd-4 and six key histone modifications (H3K4me1, H3K4me3, H3K9me3, H3K27ac, H3K27me3 and H3K36me3), as well as the transcription factor myb and the FLAG epitope. For studies that require evaluation of epitopes not contained in the preceding list, please refer to our ChIP-seq Custom Antibody Evaluation service.