Udeshi ND, Mani DC, Satpathy S, Fereshetian S, Gasser JA, Svinkina T, Olive ME, Ebert BL, Mertins P, Carr SA. Rapid and deep-scale ubiquitylation profiling for biology and translational research spectrometry. Nat Comm. 2020 Jan 17;11(1):359. PMCID: PMC6969155
Udeshi ND, Mertins P, Svinkina T, Carr SA. Large-scale identification of ubiquitination sites by mass spectrometry. Nat Protoc. 2013 Oct;8(10):1950-60. PMCID: PMC4725055
Krönke J, Udeshi ND, Narla A, Grauman P, Hurst SN, McConkey M, Svinkina T, Heckl D, Comer E, Li X, Ciarlo C, Hartman E, Munshi N, Schenone M, Schreiber SL, Carr SA, Ebert BL. Lenalidomide causes selective degradation of IKZF1 and IKZF3 in multiple myeloma cells. Science. 2014 Jan 17;343(6168):301-5. PMCID: PMC4725055
Theurillat JP*, Udeshi ND*, Errington WJ, Svinkina T, Baca SC, Pop M, Wild PJ, Blattner M, Groner AC, Rubin MA, Moch H, Prive GG, Carr SA, Garraway LA. Prostate cancer. Ubiquitylome analysis identifies dysregulation of effector substrates in SPOP-mutant prostate cancer. Science. 2014 Oct 3;346(6205):85-89. *contributed equally PMCID: PMC4257137
Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science. 2013 Mar 15;339(6125):1328-1331. doi: 10.1126/science.1230593. PMCID: PMC3916822
Udeshi ND, Pedram K, Svinkina T, Fereshetian S, Myers SA, Aygun O, Krug K, Clauser K, Ryan D, Ast T, Mootha VK, Ting AY, Carr SA. Antibodies to biotin enable large-scale detection of biotinylation sites on proteins. Nat Methods. 2017 Dec;14(12):1167-1170.
Namrata Udeshi, Ph.D.
Namrata Udeshi is a research scientist and the associate director of the Proteomics Platform at the Broad Institute of MIT and Harvard. Her focus over the past >15 years has been the development of novel state-of-the-art mass spectrometry-driven technology for analysis of protein expression, interaction, and post-translational modifications. Udeshi performs unbiased analyses at nearly whole proteome coverage to decipher signaling pathways and identify key regulatory proteins in biological systems that are associated with physiological disease or drug perturbation phenotypes.
Udeshi has a strong focus in the area of protein ubiquitination and has developed proteomic methods for enrichment and quantification of ubiquitylation sites at a global scale, to routinely detect ubiquitination sites from cell or tissue samples. The developed techniques have been critical for the identification of ubiquitin ligase substrates and have revealed key transcription factors modulated upon lenalidomide treatment in myeloma, as well as substrates targeted by recurrently mutated ligases in prostate cancer. Udeshi also uses and develops proteomic methods to identify proteins from enzyme-mediated proximity biotinylation.
Udeshi completed her Ph.D. in chemistry from the University of Virginia under the direction of Donald F Hunt. She joined the Broad Institute in 2009 as a postdoctoral associate with Steven Carr. Udeshi holds a B.A. in both dhemistry and economics from Boston University.