18.417 Lecture 9

10/12/01


Click here to start


Table of Contents

18.417 Lecture 9

Introduction

Before the Revolution

PPT Slide

Recombinant DNA operations

Cutting

Pasting

Copying

Amplifying with flanking primers

PCR: polymerase chain reaction

Making the primers

Why are all those proteins needed?

Sequencing Reaction

PPT Slide

PPT Slide

Learning where the Ts are

PPT Slide

From dyes to sequence

Sequencing a small genome

Sequencing a small genome

PPT Slide

PPT Slide

PPT Slide

PPT Slide

From plates to sequence

PPT Slide

PPT Slide

Production Details

Finishing

PPT Slide

Sequencing a Large Genome

Two levels of libraries

PPT Slide

PPT Slide

PPT Slide

PPT Slide

Next time: Assembly Now: Let’s look at the lab

Further Reading

Author: Manolis

Email: manoli@mit.edu

Home Page: http://theory.lcs.mit.edu/~bab/01-18.417-home.html

Other information:
18.417 Fall 2001 Manolis Kamvysselis For class use only