You can now limit the maximum number of threads DISCOVAR de novo uses with the new option NUM_THREADS (release 51183). This is useful if you have to share your hardware, or if your system admin has limited the number of threads a single process can use. It can also be a good idea to restrict the number of threads if your hardware has many cores (>50), as the parallelization efficiency can start to drop due to locking and cache coherency issues.
We found a serious bug in DISCOVAR de novo, revision 50862, resulting in data corruption in some cases. We will correct this bug as soon as possible. In the meantime we are rolling back to revision 50693. And needless to say, we are beefing up our release testing.
The latest release (50893) of DISCOVAR de novo now supports BAM files directly, and no longer requires SAMtools to be installed. This change has the added benefit of halving the time required to import data from a BAM, potentially saving hours on a human sized genome. Note that the original variant calling version of DISCOVAR still requires SAMtools in order to work.
Thanks to new data compression methods, our latest release (50963) reduces the peak memory required for large genomes. The exact peak memory required depends on a number of factors, including the nature of the genome and the quality of the data, but is mostly determined by coverage and genome size.
The assembly graph can be large, complex and unwieldy, so DISCOVAR de novo does not generate a viewable graph directly. Instead we have developed an interactive tool that allows you to explore your assembly by creating smaller viewable graphs of the regions you are interested in. This new tool, called NhoodInfo, is now part of the DISCOVAR package, as of release 50612 . It is also the engine behind our online demo,
so you can try it out right now without having to create an assembly of your own. Full instructions on using NhoodInfo are included in the DISCOVAR package.
We are pleased to announce the release of our new de novo assembler suitable for large genomes up to human size. This is an early release and should be considered experimental, but is fully functioning. Download it now.
Our new assembler, called DISCOVAR de novo (experimental), uses the same cheap data that the original DISCOVAR release does: 250 base paired-end PCR-free Illumina reads. No other libraries are required. The runtime for a human genome on a 48 core, 0.5 Tb server is only 36 hours, and produces an assembly with a contig N50 of ~100 kb.
We are actively developing DISCOVAR de novo, so check back often for updates.
DISCOVAR can now be freely used without restriction in both non-academic and academic settings under the terms of our new license. We still encourage users to register with us if they find DISCOVAR useful.
We’ve just added some examples to our online demo to help you explore our DISCOVAR de novo assembly of NA12878. You can select a region of interest from the new drop down menu and it will be displayed below. Alternatively, enter the coordinates of your favorite region of the genome if you want to explore on your own.