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Please note that AV454 is a module within the ARACHNE software package.  To obtain AV454 please follow the above download link to acquire the ARACHNE package.

AV454 (AssembleViral454) is an assembler, based on the ARACHNE package, designed for small and non-repetitive genomes sequenced at high depth. It was specifically designed to assemble read data generated from a mixed population of viral genomes. Reads need not be paired, and it is assumed that no sequence repeat in the genome would be large enough to fully contain an average read.

The assembly process consists of two steps: First, a pre-processing stage is run, the output of which is an initial read layout. This is identical to the process employed in the published ARACHNE algorithm. This stage generally results in a fragmented assembly. Second, we employ an iterative procedure that incrementally merges contigs and improves read placement.

Henn MR, Boutwell CL, Charlebois P, Lennon NJ, Power KA, Macalalad AR, Berlin AM, Malboeuf CM, Ryan EM, Gnerre S, Zody MC, Erlich RL, Green LM, Berical A, Wang Y, Casali M, Steeck H, Bloom AK, Dudek T, Tully D, Newman R, Axten KL, Gladden AD, Battis L, Kemper M, Zeng Q, Shea TP, Gujja S, Zedlack C, Gasser O, Brander C, Hess C, Gunthard HF, Brumme ZL, Brumme CJ, Bazner S, Rychert J, Tinsley JP, Mayer KH, Rosenberg E, Pereya F, Levin JZ, Young SK, Jessen H, Altfeld M, Birren BW, Walker BD, Allen TM(2012) Whole Genome Deep Sequencing of HIV-1 Reveals the Impact of Early Minor Variants Upon Immune Recognition During Acute Infection. PLoS Pathogens 8(3): e1002529.

Support for this tool was provided by the National Institute of Allergy and Infectious Diseases funded Microbial Sequencing Center and Genome Sequencing Center for Infectious Diseases at the Broad Institute, and by grants made by the Bill & Melinda Gates Foundation (awarded to the Ragon Institute), the NIAID-DAIDS (awarded to Ragon Institute), and the Gordon & Betty Moore Foundation, and through internal support at the Broad Institute.