Tutorials

These helpful guides are meant to educate potential collaborators about some of the technologies and methodologies utilized by the Proteomics Platform.

Proteomic Mass Spectrometry: An overview of our core technology and how we use it to identify proteins.

SILAC (Stable Isotope Labeling of Amino Acids in Culture): A quantitative technique based on metabolic labeling of cellular proteins prior to sample preparation.

iTRAQ (Isobaric Tags for Relative and Absolute Quantification): A quantitative technique based on chemical labeling of proteins after sample preparation.

Phosphoproteomics: Specific methodologies to focus on signalling and phosphorylation events.

Target Identification: Techniques to probe interactions of small molecules (like drugs) with the proteins that bind to them.

MRM (Multiple Reaction Monitoring): A focused quantitative method that uses synthetic peptides as standards for quantification of specific proteins in pre-clinical samples.

SISCAPA: An antibody-based technique used to enrich specific protein or peptide targets prior to MS-based quantification.

Proteome Fractionation: The proteome is extremely complex.  Sometimes it's best to divide and conquer!