Tutorials and Workshops
These helpful guides are meant to educate potential collaborators about some of the technologies and methodologies utilized by the Proteomics Platform.
Proteomic Mass Spectrometry: An overview of our core technology and how we use it to identify proteins.
SILAC (Stable Isotope Labeling of Amino Acids in Culture): A quantitative technique based on metabolic labeling of cellular proteins prior to sample preparation.
iTRAQ (Isobaric Tags for Relative and Absolute Quantification): A quantitative technique based on chemical labeling of proteins after sample preparation.
Phosphoproteomics: Specific methodologies to focus on signalling and phosphorylation events.
Target Identification: Techniques to probe interactions of small molecules (like drugs) with the proteins that bind to them.
MRM (Multiple Reaction Monitoring): A focused quantitative method that uses synthetic peptides as standards for quantification of specific proteins in pre-clinical samples.
SISCAPA: An antibody-based technique used to enrich specific protein or peptide targets prior to MS-based quantification.
Proteome Fractionation: The proteome is extremely complex. Sometimes it's best to divide and conquer!
Everything you always wanted to know about proteomics but were afraid to ask (Dec. 2012): This full day workshop provides a working knowledge of what proteomics is and how it can accelerate the research of biologists and clinicians.