Proteomic Mass Spectrometry Intro

Proteomics is an emerging field that has been highly enabled by the human genome project. Proteins are the products of genes, the machinery of the cells in our bodies. When genes are disrupted, the proteins are also affected. When pathogens infect us, causing disease, proteins play a key role in signaling the presence and ridding us of these invaders. Almost every process that occurs in our cells – from the metabolization of simple sugar to the division of cells – is dependent on proteins for smooth operation.

In general, proteomics seeks to detect and quantify as many proteins as possible. To do this, we often employ a very high tech method called mass spectrometry. In this tutorial, we provide an introduction to the techniques that we use at the Broad Institute to perform proteomics studies.


  1. What is mass spectrometry?
  2. Types of mass spectrometers at the Broad
  3. How we get from biological samples to protein identifications
  4. Further suggested reading