Genomics Technologies Sample Quality and Quantity Specifications

Human Exome Sequencing	Preferred DNA amount*	Required DNA concentration*
Standard Exome Sequencing	500ng*	2ng/uL
Exome Express Sequencing	500ng*	2ng/uL
Exome Plus	500ng*	2ng/uL
*Samples below 250ng (2ng/uL minimum concentration) and FFPE samples will be accepted at risk. DNA concentration as measured by PicoGreen method. Spectrophotometry (A260) is not a recommended quantification method for these products.

Whole Genome Sequencing	Preferred DNA amount*	Required DNA concentration*
Standard Light Coverage Whole Genome Sequencing (6x)	500ng	2ng/uL
Standard Light Coverage Whole Genome Sequencing (30x)	500ng	2ng/uL
Standard Light Coverage Whole Genome Sequencing (60x)	500ng	2ng/uL
Non-Human Whole Genome Sequencing	500ng	5ng/uL
*Samples below 250ng (2ng/uL minimum concentration) and FFPE samples will be accepted at risk. DNA concentration as measured by PicoGreen method. Spectrophotometry (A260) is not a recommended quantification method for these products.

RNA Sequencing	Preferred RNA amount	Required RNA concentration*
Standard RNA Sequencing – Low Coverage	500ng*	5ng/uL
Standard RNA Sequencing – High Coverage	500ng*	5ng/uL
Strand-Specific RNA Sequencing – Low Coverage	5ug**	100ng/uL
Strand-Specific RNA Sequencing – High Coverage	5ug**	100ng/uL
Samples below 250ng (2ng/uL minimum concentration) will be accepted at risk. RNA with a RIN (or equivalent QC metric) score below 7 will be attempted on risk *Samples below 5ug (100ng/uL minimum concentration) will be accepted at risk. RNA with a RIN (or equivalent QC metric) score below 7 will be attempted on risk

Whole Genome Arrays	Minimum DNA volume	Required DNA concentration*	Minimum number of samples^
Infinium Exome	30uL	50ng/uL	95
Infinium OmniExpress	30uL	50ng/uL	95
Infinium Omni2.5-8 sample	30uL	50ng/uL	95
Infinium Omni5M-quad	35uL	50ng/uL	95
Infinium OmnExpress + Exome Array	30uL	50ng/uL	95
*DNA concentration as measured by PicoGreen method. Spectrophotometry (A260) is not a recommended quantification method for these platforms. ^Minimum number of samples applies to external collaborators; contact us if you are a Broad affiliate.

Custom Content Genotyping	Minimum DNA volume	Required DNA concentration*	Minimum number of samples^
Sequenom iPlex MassArray	20uL + 5uL per pool	5ng/uL	100,000 genotypes (samples x SNPs); pools up to 7 SNPs
Sequenom hME MassArray	20uL + 5uL per pool	2.5ng/uL	100,000 genotypes (samples x SNPs); pools up to 36 SNPs
*DNA concentration as measured by PicoGreen method. Spectrophotometry (A260) is not a recommended quantification method for these platforms. ^Minimum number of samples applies to external collaborators; contact us if you are a Broad affiliate.