Genomics Technologies Sample Quality and Quantity Specifications

Human Exome Sequencing

Preferred
DNA amount* 
Required
DNA concentration*
Standard Exome Sequencing 500ng* 2ng/uL
Exome Express Sequencing 500ng* 2ng/uL
Exome Plus 500ng* 2ng/uL
*Samples below 250ng (2ng/uL minimum concentration) and FFPE samples will be accepted at risk. DNA concentration as measured by PicoGreen method. Spectrophotometry (A260) is not a recommended quantification method for these products.

 

Whole Genome Sequencing

Preferred
DNA amount* 
Required
DNA concentration*
Standard Light Coverage
Whole Genome Sequencing (6x)
500ng 2ng/uL
Standard Light Coverage
Whole Genome Sequencing (30x)
500ng 2ng/uL
Standard Light Coverage
Whole Genome Sequencing (60x)
500ng 2ng/uL
Non-Human
Whole Genome Sequencing
500ng 5ng/uL
*Samples below 250ng (2ng/uL minimum concentration) and FFPE samples will be accepted at risk. DNA concentration as measured by PicoGreen method. Spectrophotometry (A260) is not a recommended quantification method for these products.

 

RNA Sequencing

Preferred
RNA amount 
Required
RNA concentration*
Standard RNA Sequencing – Low Coverage 500ng* 5ng/uL
Standard RNA Sequencing – High Coverage 500ng* 5ng/uL
Strand-Specific RNA Sequencing – Low Coverage 5ug** 100ng/uL
Strand-Specific RNA Sequencing – High Coverage 5ug** 100ng/uL
*Samples below 250ng (2ng/uL minimum concentration) will be accepted at risk. RNA with a RIN (or equivalent QC metric) score below 7 will be attempted on risk
**Samples below 5ug (100ng/uL minimum concentration) will be accepted at risk. RNA with a RIN (or equivalent QC metric) score below 7 will be attempted on risk

 

Whole Genome Arrays

Minimum
DNA volume
Required
DNA concentration*
Minimum
number of samples
^
Infinium Exome 30uL 50ng/uL 95
Infinium OmniExpress 30uL 50ng/uL 95
Infinium Omni2.5-8 sample 30uL 50ng/uL 95
Infinium Omni5M-quad 35uL 50ng/uL 95
Infinium OmnExpress + Exome Array 30uL 50ng/uL 95
*DNA concentration as measured by PicoGreen method. Spectrophotometry (A260) is not a recommended quantification method for these platforms.
^Minimum number of samples applies to external collaborators; contact us if you are a Broad affiliate.

 

Custom Content Genotyping

Minimum
DNA volume
Required
DNA concentration*
Minimum
number of samples
^
Sequenom iPlex MassArray 20uL + 5uL per pool 5ng/uL 100,000 genotypes (samples x SNPs); pools up to 7 SNPs
Sequenom hME MassArray 20uL + 5uL per pool 2.5ng/uL 100,000 genotypes (samples x SNPs); pools up to 36 SNPs
*DNA concentration as measured by PicoGreen method. Spectrophotometry (A260) is not a recommended quantification method for these platforms.
^Minimum number of samples applies to external collaborators; contact us if you are a Broad affiliate.