Sample Quality and Quantity Specifications

Human Whole Exome Sequencing

Minimum Required
DNA amount* 
Minimum Required
DNA concentration*
Human Germline Whole Exome Sequencing 250ng 2ng/uL
Human Somatic Whole Exome Sequencing 250ng 2ng/uL
Exome Express 250ng 2ng/uL

Human Whole Genome Sequencing

Minimum Required
DNA amount* 
Minimum Required
DNA concentration*
Whole Genome Sequencing (30x) 250ng 2ng/uL
Whole Genome Sequencing (60x) 250ng 2ng/uL

Human Whole Transcriptome Sequencing

Minimum Required
RNA amount* 
Minimum Required
RNA concentration*
Standard Whole Transcriptome Sequencing
(50M pairs)
250ng 2ng/uL
Long-Insert Whole Transcriptome Sequencing (50M pairs) 250ng 2ng/uL

Human Whole Genome Arrays

Minimum Required
DNA volume
Minimum Required
DNA concentration*

Number of samples^
Infinium Exome 30uL 50ng/uL 95
Infinium OmniExpress 30uL 50ng/uL 95
Infinium Omni2.5-8 sample 30uL 50ng/uL 95
Infinium Omni5M-quad 35uL 50ng/uL 95
Infinium OmnExpress + Exome Array 30uL 50ng/uL 95

Custom Content Genotyping

Minimum Required
DNA volume
Minimum Required
DNA concentration*

Number of samples^
Sequenom iPlex MassArray

 
20uL + 5uL
per pool
 
5ng/uL

 
100,000 genotypes (samples x SNPs); pools up to 7 SNPs
Sequenom hME MassArray


 
20uL + 5uL
per pool

 
2.5ng/uL


 
100,000 genotypes (samples x SNPs); pools up to 36 SNPs
*Samples below 250ng (2ng/uL minimum concentration) and FFPE samples will be accepted at risk. DNA concentration as measured by PicoGreen method. Spectrophotometry (A260) is not a recommended quantification method for these products.
^Minimum number of samples applies to external collaborators; contact us if you are a Broad affiliate.