Illumina Infinium Whole Genome Genotyping

Illumina's BeadArray Technology is based on 3-micron silica beads that self assemble in microwells on planar silica slides. When randomly assembled on this substrate, the beads have a uniform spacing of ~5.7 microns. Each bead is covered with hundreds of thousands of copies of a specific oligonucleotide that act as the capture sequences in one of Illumina's assays.

The Genetic Analysis Platform offers off-the-shelf, fixed-content Illumina SNP genotyping assays of various scales as well as custom iSelect arrays, all of which are processed in an automated, LIMS-based production environment with customized software for accurate and automated allele calling.

Illumina Whole Genome Arrays are designed using tag SNP content, which allows for great statistical power and genomic coverage. The same Beadchips can also be used in loss of heterozygosity (LOH)/copy number analyses.

Arrays available

GAP is currently offering GWAS arrays from Illumina’s “GWAS roadmap,” which features the Omni series of arrays. This series of arrays contains updated content from the 1000 Genomes Project and these arrays allow investigators to probe up to 5 million SNPs at one time. The current arrays GAP is offering from this series include: OmniExpress, Omni1-quad, Omni2.5-quad, Omni2.5-8 sample array, Omni5M-quad and the OmniExpress + Exome array (combination array with OmniExpress and Exome array content). We also provide genotyping using the CytoSNP-12.

GAP has the ability to work with Illumina to add custom content to many of these arrays. If you are interested in custom content please indicate the array you are interested in and an approximate number of custom SNPs when you fill out the GAP inquiry form. 

For more information from Illumina about the GWAS roadmap, click here.

For more information from Illumina about all of Illumina’s whole-genome SNP arrays, click here.

GAP offers array based methylation analysis using the Infinium Methylation 450K. For more information from Illumina about methylation arrays, click here.

Minimum requirements

A minimum of 95 samples is required for Illumina whole genome arrays.