Vectors

Vector Summary (CSV)

This page displays a summary of our currently available shRNA and ORF cloning vectors. If you cannot find information about a particular vector on this page it may have been discontinued, replaced, or redesignated. Please refer to our Discontinued and Redesignated Vector Page for details.

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Constitutive shRNA Vectors

Vector Name Alias Pol II cassette 1 Pol II cassette 2 Pol III promoter Pol III insert Selection Marker Visible Reporter Cloning Site Control Clones
1 pLKO.1 pLKO_TRC001 PGK-PuroR N/A constitutive hU6 1.9 KB Stuffer PuroR N/A 1.9 KB Stuffer 101
2 pLKO_TRC005 pLKO.5 PGK-PuroR N/A constitutive hU6 1.9 KB Stuffer PuroR N/A 1.9 KB Stuffer 85
3 pLKO_TRC008 N/A PGK-BlastR 2A-tGFP constitutive hU6 1.9 KB Stuffer BlastR tGFP 1.9 KB Stuffer 2
4 pLKO_TRC016 N/A PGK-BlastR N/A constitutive hU6 1.9 KB Stuffer BlastR N/A 1.9 KB Stuffer 2
5 pLKO_TRC017 N/A PGK-NeoR N/A constitutive hU6 1.9 KB Stuffer NeoR N/A 1.9 KB Stuffer 2
6 pLKO_TRC018 N/A PGK-HygroR N/A constitutive hU6 1.9 KB Stuffer HygroR N/A 1.9 KB Stuffer 2
7 pLKO_TRC021 N/A PGK-PuroR 2A-tGFP constitutive hU6 1.9 KB Stuffer PuroR tGFP 1.9 KB Stuffer 2
8 pLKO_TRC022 N/A PGK-PuroR 2A-tRFP constitutive hU6 1.9 KB Stuffer PuroR tRFP 1.9 KB Stuffer 2
9 pLKO_TRC023 N/A PGK-PuroR 2A-mCd90 (thy1.1) constitutive hU6 1.9 KB Stuffer PuroR N/A 1.9 KB Stuffer 2
10 pLKO_TRC024 N/A PGK-BlastR 2A-mCd90 (thy1.1) constitutive hU6 1.9 KB Stuffer BlastR N/A 1.9 KB Stuffer 2
11 pLKO_TRC046 N/A PGK-BlastR 2A-eGFP constitutive hU6 1.9 KB Stuffer BlastR eGFP 1.9 KB Stuffer 2
12 pLKO_TRC047 N/A PGK-PuroR 2A-eGFP constitutive hU6 1.9 KB Stuffer PuroR eGFP 1.9 KB Stuffer 2
13 pLKO_TRC060 N/A PGK-PuroR-2A-BlastR 2A-eGFP constitutive hU6 None PuroR-2A-BlastR eGFP N/A 1
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Inducible shRNA Vectors

Vector Name Alias Pol II cassette 1 Pol II cassette 2 Pol III promoter Pol III insert Selection Marker Visible Reporter Cloning Site Control Clones
1 pLI_TRC912 pLKO_TRC912 PGK-PuroR 2A-LacI U6 inducible 1 x LacO 1.9 KB Stuffer PuroR N/A 1.9 KB Stuffer 1
2 pLI_TRC913 pLKO_TRC913 PGK-PuroR 2A-LacI U6 inducible 3 x LacO 1.9 KB Stuffer PuroR N/A 1.9 KB Stuffer 1
3 pLI_TRC950 N/A PGK-TetR 2A-PuroR H1 inducible 2 x TetO 1.9 KB Stuffer PuroR N/A 1.9 KB Stuffer 1
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Constitutive Gateway Destination ORF Vectors

Vector Name Alias Pol II cassette 1 Pol II cassette 2 Selection Marker Visible Reporter Cloning Site Epitope Tag Control Clones
1 pLX_TRC301 pLEX972new PGK-PuroR CMV-Gateway-No Tag PuroR N/A Gateway - Destination N/A 20
2 pLX_TRC302 pLEX974new PGK-PuroR CMV-Gateway-V5(c-term) PuroR N/A Gateway - Destination V5 5
3 pLX_TRC303 pLEX984new PGK-BlastR CMV-Gateway-No Tag BlastR N/A Gateway - Destination N/A 4
4 pLX_TRC304 pLEX983new PGK-BlastR CMV-Gateway-V5(c-term) BlastR N/A Gateway - Destination V5 15
5 pLX_TRC305 pCW107v2 Dest SV40-PuroR PGK-Gateway-No Tag PuroR N/A Gateway - Destination N/A 4
6 pLX_TRC306 pCW107v2 V5 Dest SV40-PuroR PGK-Gateway-V5(c-term) PuroR N/A Gateway - Destination V5 4
7 pLX_TRC307 pCW111v2 V5 Dest SV40-PuroR EF1a-Gateway-V5(c-term) PuroR N/A Gateway - Destination V5 4
8 pLX_TRC311 N/A SV40-BlastR EF1a-Gateway-V5(c-term) BlastR N/A Gateway - Destination V5 4
9 pLX_TRC312 N/A SV40-eGFP EF1a-Gateway-V5(c-term) N/A eGFP Gateway - Destination V5 3
10 pLX_TRC313 N/A SV40-HygroR EF1a-Gateway-V5(c-term) HygroR N/A Gateway - Destination V5 4
11 pLX_TRC314 N/A SV40-NeoR EF1a-Gateway-V5(c-term) NeoR N/A Gateway - Destination V5 4
12 pLX_TRC315 N/A SV40-PuroR hSyn1-Gateway-V5(c-term) PuroR N/A Gateway - Destination V5 4
13 pLX_TRC317 pLX_TRC307WBC SV40-PuroR EF1a-Gateway-V5(c-term) PuroR N/A Gateway - Destination V5 5
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Inducible Gateway Destination ORF Vectors

Vector Name Alias Pol II cassette 1 Pol II cassette 2 Selection Marker Visible Reporter Cloning Site Epitope Tag Control Clones
1 pLXI_TRC401 pCW57.1 Dest TRE-Gateway-No Tag PGK-PuroR-2A-rtTA-VP16 PuroR N/A Gateway - Destination N/A 4
2 pLXI_TRC402 pCW57v2 HA Dest TRE-Gateway-HA PGK-PuroR-2A-rtTA-VP16 PuroR N/A Gateway - Destination HA 4
3 pLXI_TRC403 pCW57v2 V5 Dest TRE-Gateway-V5 PGK-PuroR-2A-rtTA-VP16 PuroR N/A Gateway - Destination V5 4
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Cloning Gateway Donor ORF Vectors

Vector Name Alias Pol II cassette 1 Pol II cassette 2 Selection Marker Visible Reporter Cloning Site Epitope Tag Control Clones
1 pDONR201 N/A N/A N/A N/A N/A N/A N/A 0
2 pDONR221 N/A N/A N/A N/A N/A N/A N/A 3
3 pDONR223 N/A N/A N/A N/A N/A N/A N/A 12
4 pENTR/D-TOPO N/A N/A N/A N/A N/A N/A N/A 0
5 pENTR1A N/A N/A N/A N/A N/A N/A N/A 0
6 pENTR2B N/A N/A N/A N/A N/A N/A N/A 0
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