TRC Vectors

Vector Summary (CSV)

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Constitutive shRNA Vectors

Vector Name Alias Pol II cassette 1 Pol II cassette 2 Pol III promoter Pol III insert Selection Marker Visible Reporter Cloning Site Control Clones
1 pLKO.1 pLKO_TRC001 PGK-PAC N/A constitutive hU6 1.9 KB Stuffer PAC N/A 1.9 KB Stuffer 101
2 pLKO_TRC005 pLKO.5 PGK-PAC N/A constitutive hU6 1.9 KB Stuffer PAC N/A 1.9 KB Stuffer 93
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Constitutive Gateway Destination ORF Vectors

Vector Name Alias Pol II cassette 1 Pol II cassette 2 Selection Marker Visible Reporter Cloning Site Epitope Tag Control Clones
1 pLX_TRC301 pLEX972new PGK-PAC CMV-gateway-No Tag PAC N/A Gateway - Destination N/A 20
2 pLX_TRC302 pLEX974new PGK-PAC CMV-gateway-V5(c-term) PAC N/A Gateway - Destination V5 5
3 pLX_TRC303 pLEX984new PGK-Bsd CMV-gateway-No Tag Bsd N/A Gateway - Destination N/A 4
4 pLX_TRC304 pLEX983new PGK-Bsd CMV-gateway-V5(c-term) Bsd N/A Gateway - Destination V5 15
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Control Clones Formerly Referred To As Vectors

Prior Name Clone ID Other Names Vector Notes
1 pENTR-BFP BRDN0000464762 pDONR223-BFP pDONR223 N/A
2 pENTR-HcRed BRDN0000464765 pDONR223-HcRed pDONR223 N/A
3 pENTR-Luc BRDN0000464768 pDONR223-Luc pDONR223 N/A
4 pENTR-LacZ BRDN0000464771 pDONR223-LacZ pDONR223 N/A
5 pENTR-eGFP BRDN0000464774 pDONR221-eGFP pDONR221 N/A
6 pLX304-BFP ccsbBroad304_99985 N/A pLX_TRC304 N/A
7 pLX301-BFP ccsbBroad301_99985 N/A pLX_TRC301 N/A
8 pLX302-BFP BRDN0000464777 N/A pLX_TRC302 N/A
9 pLX304-HcRed ccsbBroad304_99988 N/A pLX_TRC304 N/A
10 pLX301-HcRed ccsbBroad301_99988 N/A pLX_TRC301 N/A
11 pLX302-HcRed BRDN0000464778 N/A pLX_TRC302 N/A
12 pLX304-Luc ccsbBroad304_99991 N/A pLX_TRC304 N/A
13 pLX301-Luc ccsbBroad301_99991 N/A pLX_TRC301 N/A
14 pLX302-Luc BRDN0000464779 N/A pLX_TRC302 N/A
15 pLX304-LacZ ccsbBroad304_99994 N/A pLX_TRC304 N/A
16 pLX301-LacZ ccsbBroad301_99994 N/A pLX_TRC301 N/A
17 pLX302-LacZ BRDN0000464780 N/A pLX_TRC302 N/A
18 pLX304-eGFP ccsbBroad304_99997 N/A pLX_TRC304 N/A
19 pLX301-eGFP ccsbBroad301_99997 N/A pLX_TRC301 N/A
20 pLX302-eGFP BRDN0000464781 N/A pLX_TRC302 N/A
21 pLKO_TRC025 TRCN0000241923 pLKO_TRC005-nullT pLKO_TRC005 N/A
22 pRosetta TRCN0000559420 N/A pLKO_TRC060 N/A
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