Transcript: promegaLuc.1

luciferase from Promega

Taxon:
Control
Gene:
LUCIFERASE (LUCIFERASE)
Length:
1653
CDS:
1..1653

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to promegaLuc.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other CONTROL Gene? Orig. Target Gene[?] Addgene[?]
1 TRCN0000072254 ATGTTTACTACACTCGGATAT pLKO.1 745 CDS 100% 10.800 LUCIFERASE n/a
2 TRCN0000231737 ATGTTTACTACACTCGGATAT pLKO_005 745 CDS 100% 10.800 LUCIFERASE n/a
3 TRCN0000072258 TGTCCGGTTATGTAAACAATC pLKO.1 1193 CDS 100% 10.800 LUCIFERASE n/a
4 TRCN0000231721 TGTCCGGTTATGTAAACAATC pLKO_005 1193 CDS 100% 10.800 LUCIFERASE n/a
5 TRCN0000072253 ACACTCGGATATTTGATATGT pLKO.1 754 CDS 100% 5.625 LUCIFERASE n/a
6 TRCN0000231732 ACACTCGGATATTTGATATGT pLKO_005 754 CDS 100% 5.625 LUCIFERASE n/a
7 TRCN0000072256 ACGCTGAGTACTTCGAAATGT pLKO.1 158 CDS 100% 5.625 LUCIFERASE n/a
8 TRCN0000231742 ACGCTGAGTACTTCGAAATGT pLKO_005 158 CDS 100% 5.625 LUCIFERASE n/a
9 TRCN0000072250 AGAATCGTCGTATGCAGTGAA pLKO.1 229 CDS 100% 4.950 LUCIFERASE n/a
10 TRCN0000231730 AGAATCGTCGTATGCAGTGAA pLKO_005 229 CDS 100% 4.950 LUCIFERASE n/a
11 TRCN0000072246 CAAATCACAGAATCGTCGTAT pLKO.1 221 CDS 100% 4.950 LUCIFERASE n/a
12 TRCN0000231699 CAAATCACAGAATCGTCGTAT pLKO_005 221 CDS 100% 4.950 LUCIFERASE n/a
13 TRCN0000072261 CACTCGGATATTTGATATGTG pLKO.1 755 CDS 100% 4.950 LUCIFERASE n/a
14 TRCN0000231707 CACTCGGATATTTGATATGTG pLKO_005 755 CDS 100% 4.950 LUCIFERASE n/a
15 TRCN0000072259 CGCTGAGTACTTCGAAATGTC pLKO.1 159 CDS 100% 4.950 LUCIFERASE n/a
16 TRCN0000231715 CGCTGAGTACTTCGAAATGTC pLKO_005 159 CDS 100% 4.950 LUCIFERASE n/a
17 TRCN0000072247 GAATCGTCGTATGCAGTGAAA pLKO.1 230 CDS 100% 4.950 LUCIFERASE n/a
18 TRCN0000231720 GAATCGTCGTATGCAGTGAAA pLKO_005 230 CDS 100% 4.950 LUCIFERASE n/a
19 TRCN0000072265 GTTGTGTTTGTGGACGAAGTA pLKO.1 1546 CDS 100% 4.950 LUCIFERASE n/a
20 TRCN0000231727 GTTGTGTTTGTGGACGAAGTA pLKO_005 1546 CDS 100% 4.950 LUCIFERASE n/a
21 TRCN0000072248 AGTCAAGTAACAACCGCGAAA pLKO.1 1510 CDS 100% 4.050 LUCIFERASE n/a
22 TRCN0000231718 AGTCAAGTAACAACCGCGAAA pLKO_005 1510 CDS 100% 4.050 LUCIFERASE n/a
23 TRCN0000072252 ACTTACGCTGAGTACTTCGAA pLKO.1 154 CDS 100% 3.000 LUCIFERASE n/a
24 TRCN0000231733 ACTTACGCTGAGTACTTCGAA pLKO_005 154 CDS 100% 3.000 LUCIFERASE n/a
25 TRCN0000072244 ATCACAGAATCGTCGTATGCA pLKO.1 224 CDS 100% 3.000 LUCIFERASE n/a
26 TRCN0000231695 ATCACAGAATCGTCGTATGCA pLKO_005 224 CDS 100% 3.000 LUCIFERASE n/a
27 TRCN0000072255 TCTACTGGTCTGCCTAAAGGT pLKO.1 601 CDS 100% 3.000 LUCIFERASE n/a
28 TRCN0000231734 TCTACTGGTCTGCCTAAAGGT pLKO_005 601 CDS 100% 3.000 LUCIFERASE n/a
29 TRCN0000072262 CACTTACGCTGAGTACTTCGA pLKO.1 153 CDS 100% 2.640 LUCIFERASE n/a
30 TRCN0000231736 CACTTACGCTGAGTACTTCGA pLKO_005 153 CDS 100% 2.640 LUCIFERASE n/a
31 TRCN0000072260 CAGAATCGTCGTATGCAGTGA pLKO.1 228 CDS 100% 2.640 LUCIFERASE n/a
32 TRCN0000231714 CAGAATCGTCGTATGCAGTGA pLKO_005 228 CDS 100% 2.640 LUCIFERASE n/a
33 TRCN0000072243 CTTCGAAATGTCCGTTCGGTT pLKO.1 168 CDS 100% 2.640 LUCIFERASE n/a
34 TRCN0000231693 CTTCGAAATGTCCGTTCGGTT pLKO_005 168 CDS 100% 2.640 LUCIFERASE n/a
35 TRCN0000072266 GAATGTTTACTACACTCGGAT pLKO.1 743 CDS 100% 2.640 LUCIFERASE n/a
36 TRCN0000231729 GAATGTTTACTACACTCGGAT pLKO_005 743 CDS 100% 2.640 LUCIFERASE n/a
37 TRCN0000072251 GAGTACTTCGAAATGTCCGTT pLKO.1 163 CDS 100% 2.640 LUCIFERASE n/a
38 TRCN0000231728 GAGTACTTCGAAATGTCCGTT pLKO_005 163 CDS 100% 2.640 LUCIFERASE n/a
39 TRCN0000072264 GCTGAGTACTTCGAAATGTCC pLKO.1 160 CDS 100% 2.640 LUCIFERASE n/a
40 TRCN0000231741 GCTGAGTACTTCGAAATGTCC pLKO_005 160 CDS 100% 2.640 LUCIFERASE n/a
41 TRCN0000072245 TCACAGAATCGTCGTATGCAG pLKO.1 225 CDS 100% 2.640 LUCIFERASE n/a
42 TRCN0000231697 TCACAGAATCGTCGTATGCAG pLKO_005 225 CDS 100% 2.640 LUCIFERASE n/a
43 TRCN0000072263 AGTACTTCGAAATGTCCGTTC pLKO.1 164 CDS 100% 2.250 LUCIFERASE n/a
44 TRCN0000231739 AGTACTTCGAAATGTCCGTTC pLKO_005 164 CDS 100% 2.250 LUCIFERASE n/a
45 TRCN0000072249 GCGGTTGCCAAGAGGTTCCAT pLKO.1 976 CDS 100% 1.000 LUCIFERASE n/a
46 TRCN0000231719 GCGGTTGCCAAGAGGTTCCAT pLKO_005 976 CDS 100% 1.000 LUCIFERASE n/a
47 TRCN0000072267 GCGCCATTCTATCCGCTGGAA pLKO.1 34 CDS 100% 0.880 LUCIFERASE n/a
48 TRCN0000231731 GCGCCATTCTATCCGCTGGAA pLKO_005 34 CDS 100% 0.880 LUCIFERASE n/a
49 TRCN0000072257 TGAGTACTTCGAAATGTCCGT pLKO.1 162 CDS 100% 0.660 LUCIFERASE n/a
50 TRCN0000231740 TGAGTACTTCGAAATGTCCGT pLKO_005 162 CDS 100% 0.660 LUCIFERASE n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript promegaLuc.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 BRDN0000464768 pDONR223 0% 100% 100% None n/a
2 ccsbBroad301_99991 pLX_301 0% 100% 100% None n/a
3 ccsbBroad301_99993 pLX_301 0% 100% 100% None n/a
4 ccsbBroad301_99982 pLX_301 0% 100% 100% None n/a
5 ccsbBroad301_99992 pLX_301 0% 100% 100% None n/a
6 ccsbBroad304_99991 pLX_304 18.9% 100% 100% V5 n/a
7 ccsbBroad304_99992 pLX_304 18.9% 100% 100% V5 n/a
8 BRDN0000556262 pLX_305 0% 100% 100% None n/a
9 BRDN0000556299 pLX_306 0% 100% 100% V5 n/a
10 BRDN0000556280 pLXI_401 0% 100% 100% None n/a
11 BRDN0000556301 pLX_311 0% 100% 100% V5 n/a
12 BRDN0000556296 pLX_312 0% 100% 100% V5 n/a
13 BRDN0000556302 pLX_313 0% 100% 100% V5 n/a
14 BRDN0000556293 pLX_314 0% 100% 100% V5 n/a
15 BRDN0000556270 pLX_315 0% 100% 100% V5 n/a
16 BRDN0000556289 pLXI_403 0% 100% 100% V5 n/a
17 BRDN0000559468 CTAATATACAACCGATTAATCATC pLX_317 30.3% 100% 100% V5 n/a
18 ccsbBroad304_99993 pLX_304 44.2% 76.8% 56.5% V5 (not translated due to prior stop codon) (many diffs) n/a
19 BRDN0000464769 pDONR223 0% 100% 100% None n/a
20 BRDN0000464770 pDONR223 0% 100% 100% None n/a
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