Transcript: lacZ.1

Identifier:
NCBI 
Description:
ecoli lacZ NC_004431:448924..451998 ncbiGeneId: 1036228
Taxon:
CONTROL
Gene:
NCBI lacZ
Length:
3074
CDS:
1..3074

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to lacZ.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other CONTROL Gene? Orig. Target Gene[?]
1 TRCN0000072236 CCAACGTGACCTATCCCATTA pLKO.1 305 CDS 100% 10.800 N/A N/A lacZ
2 TRCN0000231735 CCAACGTGACCTATCCCATTA pLKO_TRC005 305 CDS 100% 10.800 N/A N/A lacZ
3 TRCN0000072242 GTCGGCTTACGGCGGTGATTT pLKO.1 1758 CDS 100% 4.400 N/A N/A lacZ
4 TRCN0000231706 GTCGGCTTACGGCGGTGATTT pLKO_TRC005 1758 CDS 100% 4.400 N/A N/A lacZ
5 TRCN0000072235 CCGTCATAGCGATAACGAGTT pLKO.1 1935 CDS 100% 4.050 N/A N/A lacZ
6 TRCN0000231738 CCGTCATAGCGATAACGAGTT pLKO_TRC005 1935 CDS 100% 4.050 N/A N/A lacZ
7 TRCN0000072233 CGACCACGCAAATCAGCGATT pLKO.1 656 CDS 100% 4.050 N/A N/A lacZ
8 TRCN0000231716 CGACCACGCAAATCAGCGATT pLKO_TRC005 656 CDS 100% 4.050 N/A N/A lacZ
9 TRCN0000072238 GTTCCGTCATAGCGATAACGA pLKO.1 1932 CDS 100% 3.000 N/A N/A lacZ
10 TRCN0000231685 GTTCCGTCATAGCGATAACGA pLKO_TRC005 1932 CDS 100% 3.000 N/A N/A lacZ
11 TRCN0000072226 CGATCGTAATCACCCGAGTGT pLKO.1 1341 CDS 100% 2.640 N/A N/A lacZ
12 TRCN0000231709 CGATCGTAATCACCCGAGTGT pLKO_TRC005 1341 CDS 100% 2.640 N/A N/A lacZ
13 TRCN0000072232 CGTCGTATTACAACGTCGTGA pLKO.1 27 CDS 100% 2.640 N/A N/A lacZ
14 TRCN0000231717 CGTCGTATTACAACGTCGTGA pLKO_TRC005 27 CDS 100% 2.640 N/A N/A lacZ
15 TRCN0000072240 TCGTATTACAACGTCGTGACT pLKO.1 29 CDS 100% 2.640 N/A N/A lacZ
16 TRCN0000231702 TCGTATTACAACGTCGTGACT pLKO_TRC005 29 CDS 100% 2.640 N/A N/A lacZ
17 TRCN0000072231 CGCTAAATACTGGCAGGCGTT pLKO.1 1650 CDS 100% 2.160 N/A N/A lacZ
18 TRCN0000231710 CGCTAAATACTGGCAGGCGTT pLKO_TRC005 1650 CDS 100% 2.160 N/A N/A lacZ
19 TRCN0000072239 GCCGTCGTATTACAACGTCGT pLKO.1 25 CDS 100% 2.160 N/A N/A lacZ
20 TRCN0000231700 GCCGTCGTATTACAACGTCGT pLKO_TRC005 25 CDS 100% 2.160 N/A N/A lacZ
21 TRCN0000072234 CGGATTCTCTGGCCGTCGTAT pLKO.1 14 CDS 100% 1.650 N/A N/A lacZ
22 TRCN0000072229 GCGATCGTAATCACCCGAGTG pLKO.1 1340 CDS 100% 0.750 N/A N/A lacZ
23 TRCN0000231712 GCGATCGTAATCACCCGAGTG pLKO_TRC005 1340 CDS 100% 0.750 N/A N/A lacZ
24 TRCN0000072224 CGCGATCGTAATCACCCGAGT pLKO.1 1339 CDS 100% 0.720 N/A N/A lacZ
25 TRCN0000231722 CGCGATCGTAATCACCCGAGT pLKO_TRC005 1339 CDS 100% 0.720 N/A N/A lacZ
26 TRCN0000072225 CTCTGGCTAACGGTACGCGTA pLKO.1 2083 CDS 100% 0.720 N/A N/A lacZ
27 TRCN0000072241 GCGTTGGCAATTTAACCGCCA pLKO.1 2265 CDS 100% 0.540 N/A N/A lacZ
28 TRCN0000231704 GCGTTGGCAATTTAACCGCCA pLKO_TRC005 2265 CDS 100% 0.540 N/A N/A lacZ
29 TRCN0000072223 TGTTCGCATTATCCGAACCAT pLKO.1 1168 CDS 100% 0.300 N/A N/A lacZ
30 TRCN0000231726 TGTTCGCATTATCCGAACCAT pLKO_TRC005 1168 CDS 100% 0.300 N/A N/A lacZ
31 TRCN0000072228 ACTCTGGCTAACGGTACGCGT pLKO.1 2082 CDS 100% 0.220 N/A N/A lacZ
32 TRCN0000231713 ACTCTGGCTAACGGTACGCGT pLKO_TRC005 2082 CDS 100% 0.220 N/A N/A lacZ
33 TRCN0000072230 CCCGTCAGTATCGGCGGAATT pLKO.1 3003 CDS 100% 0.000 N/A N/A lacZ
34 TRCN0000231711 CCCGTCAGTATCGGCGGAATT pLKO_TRC005 3003 CDS 100% 0.000 N/A N/A lacZ
35 TRCN0000072237 CGCGCCTTTCGGCGGTGAAAT pLKO.1 816 CDS 100% 0.000 N/A N/A lacZ
36 TRCN0000231743 CGCGCCTTTCGGCGGTGAAAT pLKO_TRC005 816 CDS 100% 0.000 N/A N/A lacZ
37 TRCN0000072227 GCGCTAATCACGACGCGCTGT pLKO.1 1397 CDS 100% 0.000 N/A N/A lacZ
38 TRCN0000231708 GCGCTAATCACGACGCGCTGT pLKO_TRC005 1397 CDS 100% 0.000 N/A N/A lacZ
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript lacZ.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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shRNA Candidate Sequences

Show high scoring hairpin designs for this transcript.

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Note
1 BRDN0000464771 pDONR223 0% 91.7% 92.2% None (many diffs)
2 ccsbBroad301_99994 pLX_TRC301 0% 91.7% 92.2% None (many diffs)
3 ccsbBroad301_99995 pLX_TRC301 0% 91.7% 92.2% None (many diffs)
4 ccsbBroad301_99996 pLX_TRC301 0% 91.7% 92.2% None (many diffs)
5 ccsbBroad301_99983 pLX_TRC301 0% 91.7% 92.2% None (many diffs)
6 ccsbBroad304_99994 pLX_TRC304 3.4% 91.7% 92.2% V5 (many diffs)
7 ccsbBroad304_99996 pLX_TRC304 3.4% 91.7% 92.2% V5 (many diffs)
8 ccsbBroad304_99995 pLX_TRC304 3.4% 91.7% 92.2% V5 (many diffs)
9 BRDN0000559441 pLX_TRC307 0% 91.7% 92.2% V5 (many diffs)
10 BRDN0000464780 pLX_TRC302 0% 91.7% 92.2% V5 (many diffs)
11 BRDN0000556272 pLX_TRC303 0% 91.7% 92.2% None (many diffs)
12 BRDN0000556278 pLX_TRC305 0% 91.7% 92.2% None (many diffs)
13 BRDN0000556288 pLX_TRC306 0% 91.7% 92.2% V5 (many diffs)
14 BRDN0000556266 pLXI_TRC401 0% 91.7% 92.2% None (many diffs)
15 BRDN0000556290 pLXI_TRC402 0% 91.7% 92.2% HA (many diffs)
16 BRDN0000556291 pLX_TRC311 0% 91.7% 92.2% V5 (many diffs)
17 BRDN0000556274 pLX_TRC312 0% 91.7% 92.2% V5 (many diffs)
18 BRDN0000556300 pLX_TRC313 0% 91.7% 92.2% V5 (many diffs)
19 BRDN0000556279 pLX_TRC314 0% 91.7% 92.2% V5 (many diffs)
20 BRDN0000556294 pLX_TRC315 0% 91.7% 92.2% V5 (many diffs)
21 BRDN0000556277 pLXI_TRC403 0% 91.7% 92.2% V5 (many diffs)
22 BRDN0000464772 pDONR223 0% 91.7% 92.2% None (many diffs)
23 BRDN0000464773 pDONR223 0% 91.7% 92.2% None (many diffs)
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