Transcript: Mouse NM_146157.4

Mus musculus ER membrane protein complex subunit 1 (Emc1), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-04-23
Taxon:
Mus musculus (mouse)
Gene:
Emc1 (230866)
Length:
6237
CDS:
34..3027

Additional Resources:

NCBI RefSeq record:
NM_146157.4
NBCI Gene record:
Emc1 (230866)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_146157.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000072440 GCAGAGCGATTCATCAACTAT pLKO.1 2761 CDS 100% 5.625 7.875 N EMC1 n/a
2 TRCN0000298203 GCAGAGCGATTCATCAACTAT pLKO_005 2761 CDS 100% 5.625 7.875 N EMC1 n/a
3 TRCN0000202444 GCGTTAAATTCTCGGACTGGA pLKO.1 229 CDS 100% 2.640 3.696 N Emc1 n/a
4 TRCN0000438262 TTTACCCGTCCAAGCAGTTTG pLKO_005 2885 CDS 100% 10.800 8.640 N Emc1 n/a
5 TRCN0000201403 GCGATTCATCAACTATAACCA pLKO.1 2766 CDS 100% 3.000 2.400 N Emc1 n/a
6 TRCN0000436414 CCACTCCCTGTTCCCTTTATG pLKO_005 3245 3UTR 100% 13.200 9.240 N Emc1 n/a
7 TRCN0000420187 GAAATATTCTGACACCTTATT pLKO_005 3411 3UTR 100% 13.200 9.240 N Emc1 n/a
8 TRCN0000414686 GCAGTATCTGCCCAATGTTAA pLKO_005 1719 CDS 100% 13.200 9.240 N Emc1 n/a
9 TRCN0000437517 AGGAGTCAGTGAGGTACATTG pLKO_005 446 CDS 100% 10.800 7.560 N Emc1 n/a
10 TRCN0000190419 CCAGTCATGGATCAAGACTAT pLKO.1 1933 CDS 100% 4.950 3.465 N Emc1 n/a
11 TRCN0000190683 GCAGTATGTTGGGAAGATCAA pLKO.1 138 CDS 100% 4.950 3.465 N Emc1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_146157.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02717 pDONR223 100% 87.9% 93% None (many diffs) n/a
2 ccsbBroad304_02717 pLX_304 0% 87.9% 93% V5 (many diffs) n/a
3 TRCN0000478824 GAGCCTGCCCGTACACACACTCTA pLX_317 11.8% 87.9% 93% V5 (many diffs) n/a
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