Transcript: Mouse NM_026042.3

Mus musculus mediator complex subunit 29 (Med29), mRNA.

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

shRNA constructs matching this transcript with 100% SDR[?]The SDR or "Specificity-Defining Region" encompasses the 19 bases within the shRNA stem region that are retained during siRNA processing/production. These are thus the bases that define the target specificity of the shRNA. Note that, while our shRNA designs nearly always extend the stem by two additional bases matching the intended target transcript (reported in the "Target Seq" column), these additional bases are not relevant to target specificity. match

This list includes all shRNAs that have a perfect SDR[?]The SDR or "Specificity-Defining Region" encompasses the 19 bases within the shRNA stem region that are retained during siRNA processing/production. These are thus the bases that define the target specificity of the shRNA. Note that, while our shRNA designs nearly always extend the stem by two additional bases matching the intended target transcript (reported in the "Target Seq" column), these additional bases are not relevant to target specificity. match to Mouse NM_026042.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

	Clone ID	Target Seq	Vector	Match Position	Match Region[?]The region of the transcript where the match occurs (5UTR, CDS, 3UTR). NOTE: all matches to non-coding transcripts are labeled 3UTR.	SDR Match %[?]Percent match of the "Specificity-Defining Region" of the hairpin target sequence to transcript RNA sequence. The SDR is defined as the initial 19 bases of the (21mer) target sequence. 84% = 16/19 89% = 17/19 95% = 18/19 100% = 19/19	Intrinsic Score[?]Also called "original score", this assesses the target sequence for predicted cloneability and knockdown performance.	Adjusted Score[?]Also called "specificity score", this is a function of the intrinsic score and the specificity factor.	Matches Other Mouse Gene?[?]Does this construct also match another Mouse gene an equal or better degree?	Orig. Target Gene[?]The gene that this construct was originally designed to target	Addgene[?]Link to this entity's page in the Addgene catalog, if available
1	TRCN0000297563	TTCCCACTGGGCCGATGATTA	pLKO_005	829	3UTR	100%	13.200	10.560	N	Med29	n/a
2	TRCN0000191677	GATCGTATTATACAAGAGGAA	pLKO.1	1022	3UTR	100%	2.640	2.112	N	Med29	n/a
3	TRCN0000278833	AGTACCTGGCTGTCATCAAAG	pLKO_005	500	CDS	100%	10.800	7.560	N	Med29	n/a
4	TRCN0000297562	CTGTGCCAAAGACATTCATAC	pLKO_005	531	CDS	100%	10.800	7.560	N	Med29	n/a
5	TRCN0000278777	TGATTCAGAACACTAACATTG	pLKO_005	269	CDS	100%	10.800	7.560	N	Med29	n/a
6	TRCN0000201589	CCCAGTGTATGGATTTCCTTT	pLKO.1	929	3UTR	100%	4.950	3.465	N	Med29	n/a
7	TRCN0000202465	GCAGCGCTTTGACAAGTGTTT	pLKO.1	321	CDS	100%	4.950	3.465	N	Med29	n/a
8	TRCN0000278832	GCAGCGCTTTGACAAGTGTTT	pLKO_005	321	CDS	100%	4.950	3.465	N	Med29	n/a
9	TRCN0000202252	GCCACAGTAATGCAGCTGAAA	pLKO.1	703	3UTR	100%	4.950	3.465	N	Med29	n/a

Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?]The SDR or "Specificity-Defining Region" encompasses the 19 bases within the shRNA stem region that are retained during siRNA processing/production. These are thus the bases that define the target specificity of the shRNA. Note that, while our shRNA designs nearly always extend the stem by two additional bases matching the intended target transcript (reported in the "Target Seq" column), these additional bases are not relevant to target specificity. match to the transcript NM_026042.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Download CSV