Transcript: Human NM_001982.3

Homo sapiens erb-b2 receptor tyrosine kinase 3 (ERBB3), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-06-04
Taxon:
Homo sapiens (human)
Gene:
ERBB3 (2065)
Length:
5765
CDS:
277..4305

Additional Resources:

NCBI RefSeq record:
NM_001982.3
NBCI Gene record:
ERBB3 (2065)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149441 ATCATGTGAGACAACACCGG pXPR_003 GGG 2406 60% 20 0.6997 ERBB3 ERBB3 77480
2 BRDN0001147186 ATGAGGCGATACTTGGAACG pXPR_003 GGG 2045 51% 17 0.4149 ERBB3 ERBB3 77479
3 BRDN0001147088 TGTCGAAATTATAGCCGAGG pXPR_003 AGG 1574 39% 13 -0.4795 ERBB3 ERBB3 77481
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001982.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000000622 CGACTAGACATCAAGCATAAT pLKO.1 1717 CDS 100% 13.200 18.480 N ERBB3 n/a
2 TRCN0000194972 CTTCGTCATGTTGAACTATAA pLKO.1 627 CDS 100% 13.200 18.480 N ERBB3 n/a
3 TRCN0000230092 TACTAAACTTCACCTACATTA pLKO_005 4845 3UTR 100% 13.200 18.480 N ERBB3 n/a
4 TRCN0000230091 TTCGTCATGTTGAACTATAAC pLKO_005 628 CDS 100% 13.200 18.480 N ERBB3 n/a
5 TRCN0000040111 GCGACTAGACATCAAGCATAA pLKO.1 1716 CDS 100% 10.800 15.120 N ERBB3 n/a
6 TRCN0000018327 TATATGAATCGGCAACGAGAT pLKO.1 4060 CDS 100% 4.050 5.670 N ERBB3 n/a
7 TRCN0000374294 CCCTTGAGAGTATCCACTTTG pLKO_005 2912 CDS 100% 10.800 8.640 N Erbb3 n/a
8 TRCN0000040112 GCCAAGGGAATGTACTACCTT pLKO.1 2731 CDS 100% 3.000 2.400 N ERBB3 n/a
9 TRCN0000218392 TGCAGCTTGTCACTCAATATT pLKO_005 2624 CDS 100% 15.000 10.500 N ERBB3 n/a
10 TRCN0000199993 CCACGGTATCTGGTCATAAAG pLKO.1 3193 CDS 100% 13.200 9.240 N ERBB3 n/a
11 TRCN0000040110 CCAGAGCTTCAAGACTGTTTA pLKO.1 2146 CDS 100% 13.200 9.240 N ERBB3 n/a
12 TRCN0000199364 CCTGTGCATGTGCTCTTATTG pLKO.1 5297 3UTR 100% 13.200 9.240 N ERBB3 n/a
13 TRCN0000219020 GACTAGACATCAAGCATAATC pLKO_005 1718 CDS 100% 13.200 9.240 N ERBB3 n/a
14 TRCN0000218445 GTCTACAACAAGCTAACTTTC pLKO_005 1066 CDS 100% 10.800 7.560 N ERBB3 n/a
15 TRCN0000000620 CCCAGTGAGAAGGCTAACAAA pLKO.1 2350 CDS 100% 5.625 3.938 N ERBB3 n/a
16 TRCN0000009835 GAGGTTAGGAGTAGATATTGA pLKO.1 4783 3UTR 100% 5.625 3.938 N ERBB3 n/a
17 TRCN0000000621 CCTGACAAGATGGAAGTAGAT pLKO.1 1195 CDS 100% 4.950 3.465 N ERBB3 n/a
18 TRCN0000000623 CTGCCTTTGATAACCCTGATT pLKO.1 4238 CDS 100% 4.950 3.465 N ERBB3 n/a
19 TRCN0000040109 GCCTACCAGTTGGAACACTTA pLKO.1 3371 CDS 100% 4.950 3.465 N ERBB3 n/a
20 TRCN0000040108 GCTCTGATACTTGTGCTCAAT pLKO.1 1982 CDS 100% 4.950 3.465 N ERBB3 n/a
21 TRCN0000010344 GAATTCTCTACTCTACCATTG pLKO.1 553 CDS 100% 0.000 0.000 N ERBB3 n/a
22 TRCN0000000619 GCTCTTATGTGTGCCTTTGTT pLKO.1 4648 3UTR 100% 5.625 3.375 N ERBB3 n/a
23 TRCN0000365981 GAGGCCAAGACTCCAATTAAA pLKO_005 2884 CDS 100% 15.000 10.500 N Erbb3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001982.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14632 pDONR223 0% 100% 100% None n/a
2 TRCN0000480432 CTGACGCAGTTTGTCTGTGAGAGG pLX_317 8.6% 99.9% 100% V5 4026delG n/a
3 ccsbBroad304_14632 pLX_304 11.8% 99.9% 99.9% V5 3706G>A;3804T>C;4026delG n/a
4 TRCN0000489936 ATCGTACGAATTATGCCTGTGTGC pLX_317 10.8% 99.9% 100% V5 (not translated due to prior stop codon) 3348G>A n/a
5 TRCN0000491817 GCTTTGGTTCCCATGATTGGGTGG pLX_317 8.5% 99.9% 99.9% V5 3348G>A;4026_4027insG n/a
6 ccsbBroadEn_10806 pDONR223 100% 24.6% 24.5% None 992G>T;994_4026del n/a
7 ccsbBroad304_10806 pLX_304 0% 24.6% 24.5% V5 992G>T;994_4026del n/a
8 TRCN0000481555 AGGCGAGCCAACTGCTTGACAGGT pLX_317 53.7% 24.6% 24.5% V5 992G>T;994_4026del n/a
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