Protocols

Informational Guides

Guide Date Link
1 Selecting a Genome-wide Library 20 Jan 2023
2 Ordering Lentiviral Libraries 27 Jan 2023

Video Protocols

Video Topic Date Link
1 Creation of a Pooled Library 27 Nov 2017
2 Large Scale Virus Infection 27 Nov 2017
3 Viral Titration 27 Nov 2017
4 Pooling Large Scale Infections 27 Nov 2017

Cloning and Plasmid DNA (pDNA) Production

Protocol Name Date Download
1 shRNA/sgRNA Cloning Protocol 30 Nov 2019
2 shRNA/sgRNA/ORF Glycerol and Plasmid DNA Preparation 30 Sep 2015
3 pDNA Library Amplification 27 Oct 2021

Virus Production and Titering

Protocol Name Date Download
1 shRNA/sgRNA/ORF High Throughput Viral Production (96 well) 9 Jan 2018
2 shRNA/sgRNA/ORF Low Throughput Viral Production (10cm dish/6 well) 3 Jun 2015
3 Lentiviral Production in Flasks 27 Nov 2023
4 Viral Titering Protocol (alamarBlue) 21 Sep 2016
5 Puromycin, Blasticidin and Hygromycin Titration Protocol 23 Sep 2020
6 Lentivirus Concentration Protocol 11 Feb 2019

Cas9 Assessment

Protocol Name Date Download
1 Cas9 detection using flow cytometry 01 Feb 2019
2 Cas9/Cas12a Activity Assay 23 Sep 2020
3 dCas9 Activity Assay for CRISPRa Screening 23 Sep 2020
4 dCas9 Activity Assay for CRISPRi Screening 23 Sep 2020
5 Out-of-frame eGFP CRISPR Gene Editing Reporter Assay 30 Sep 2015

Viral Infection

Protocol Name Date Download
1 Optimization of Lentiviral Spinfection 23 Sep 2020
2 No-Spin Infection Protocol 11 Feb 2019

gDNA Isolation, PCR and Next-Generation Sequence Data Analysis

Protocol Name Date Download
1 gDNA Isolation Protocol with NucleoSpin Blood Kits and PCR pre-check 09 Apr 2021
2 sgRNA/shRNA/ORF PCR for Illumina Sequencing 22 Jan 2024
3 Pooled Screening Deconvolution Using PoolQ (old version: PoolQ 2.x) 20 Dec 2019
4 Pooled Screening Deconvolution Using PoolQ (current version: PoolQ 3.x) 20 Dec 2019
5 Pooled Screen Analysis Guide 22 Oct 2020