TRC Protocols

TRC Library Production and Performance Protocols

Protocol Name Date Download
1 shRNA Cloning Protocol 13 Feb 2013 pdf
2 shRNA and ORF Glycerol and Plasmid DNA Preparation 5 Dec 2012 pdf
3 shRNA/sgRNA/ORF High Throughput Viral Production (96 well) 3 Jun 2015 pdf
4 shRNA/sgRNA/ORF Low Throughput Viral Production (10cm dish/6 well) 3 Jun 2015 pdf
5 Lentiviral Infection Protocol 13 Dec 2012 pdf
6 Viral Titering Protocol (alamarBlue) 3 Jun 2015 pdf
7 TRC Knockdown Validation with qPCR 5 Dec 2012 pdf
8 TRC Assay Development Guidelines for ORF clones 4 Feb 2011 pdf
9 shRNA Plasmid DNA/Viral Pool Production Protocol 5 Dec 2012 pdf
10 V5 Tag Staining Protocol 5 Dec 2012 pdf
11 Mycoplasma Detection Protocol 23 Apr 2014 pdf

Pooled Screening Protocols

Protocol Name Date Download
1 Large scale infection and tissue culture protocol 9 Feb 2009 doc
2 Sample and microarray processing 9 Feb 2009 doc
3 dChip preprocessing protocol 9 Feb 2009 doc
4 Illumina Sequencing Nested Barcode PCR Protocol 5 Dec 2012 pdf
5 Illumina Sequencing One Step Barcode PCR 5 Dec 2012 pdf