TRC Protocols

TRC Library Production and Performance Protocols

Protocol Name Date Download
1 Glycerol stock and plasmid preparation 22 Jan 2007 pdf
2 Lentivirus production 9 Aug 2010 pdf
3 Lentivirus infection 9 Feb 2009 pdf
4 Relative viral titering with resazurin (alamarBlue), puromycin selection 24 Mar 2012 pdf
5 Relative viral titering with resazurin (alamarBlue), blasticidin selection 24 Mar 2012 pdf
6 TRC Knockdown Validation with qPCR 31 May 2010 pdf
7 TRC Assay Development Guidelines for ORF clones 04 Feb 2011 pdf
8 ORF Glycerol Handling and DNA Preparation Protocol 08 Sep 2011 pdf
9 ORF Virus Production Protocol 08 Sep 2011 pdf
10 ORF AlamarBlue Titering Protocol 08 Sep 2011 pdf

Pooled Screening Protocols

Protocol Name Date Download
1 Large scale infection and tissue culture protocol 9 Feb 2009 doc
2 Sample and microarray processing 9 Feb 2009 doc
3 dChip preprocessing protocol 9 Feb 2009 doc