TRC Protocols

TRC Library Production and Performance Protocols

Protocol Name Date Download
1 shRNA Cloning Protocol 13 Feb 2013 pdf
2 shRNA and ORF Glycerol and Plasmid DNA Preparation 5 Dec 2012 pdf
3 shRNA and ORF Viral Production 5 Dec 2012 pdf
4 Lentiviral Infection Protocol 13 Dec 2012 pdf
5 Viral Titering Protocol (alamarBlue) 5 Dec 2012 pdf
6 TRC Knockdown Validation with qPCR 5 Dec 2012 pdf
7 TRC Assay Development Guidelines for ORF clones 4 Feb 2011 pdf
8 shRNA Plasmid DNA/Viral Pool Production Protocol 5 Dec 2012 pdf
9 V5 Tag Staining Protocol 5 Dec 2012 pdf
10 Mycoplasma Detection Protocol 23 Apr 2014 pdf

Pooled Screening Protocols

Protocol Name Date Download
1 Large scale infection and tissue culture protocol 9 Feb 2009 doc
2 Sample and microarray processing 9 Feb 2009 doc
3 dChip preprocessing protocol 9 Feb 2009 doc
4 Illumina Sequencing Nested Barcode PCR Protocol 5 Dec 2012 pdf
5 Illumina Sequencing One Step Barcode PCR 5 Dec 2012 pdf