Protocols

Library Production, Performance and Pooled Screening Protocols

Protocol Name Date Download
1 gDNA Miniprep Isolation Protocol 21 Jan 2016 pdf
2 gDNA Midi/Maxiprep Isolation Protocol 21 Jan 2016 pdf
3 sgRNA/shRNA/ORF PCR for Illumina Sequencing 26 Oct 2015 pdf
4 shRNA/sgRNA Cloning Protocol 30 Sep 2015 pdf
5 shRNA/sgRNA/ORF Glycerol and Plasmid DNA Preparation 30 Sep 2015 pdf
6 shRNA/sgRNA/ORF High Throughput Viral Production (96 well) 30 Sep 2015 pdf
7 Viral Titering Protocol (alamarBlue) 30 Sep 2015 pdf
8 Cas9 Activity Assay 30 Sep 2015 pdf
9 Introducing Cas9 Lentivirus 30 Sep 2015 pdf
10 Out-of-frame eGFP CRISPR Gene Editing Reporter Assay 30 Sep 2015 pdf
11 shRNA/sgRNA/ORF Low Throughput Viral Production (10cm dish/6 well) 3 Jun 2015 pdf
12 Mycoplasma Detection Protocol 23 Apr 2014 pdf